Methods and materials for development of high-throughput screening assays for detection of cyclic GMP (cGAMP) are provided by this invention.

Methods and materials for development of high-throughput screening assays for detection of cyclic GMP (cGAMP) are provided by this invention.

The present disclosure relates to lateral flow methods and apparatuses for detecting one or more analytes. Certain embodiments provide a lateral flow device, kit and method of using the same, comprising: a flow path defined by a permeable sub-assembly of the lateral flow device, a release zone comprising a plurality of peptide-tagged agents, and a detection zone comprising a plurality of anti-peptide agents present in the flow path at a ratio of at least 100:1, on a weight:weight basis, relative to the plurality of peptide-tagged agents.

The present invention relates to aptamer-based assays to capture and/or detect analytes comprising primary and secondary aptamers, as well as compositions comprising such primary and secondary aptamers, wherein analytes comprise small molecules that offer limited mutually non-competitive epitopes to antibodies, that is, with limited ability to measure in non-competitive sandwich assays using primary and secondary antibodies or primary and secondary aptamers.

The present invention relates to aptamer-based assays to capture and/or detect analytes comprising primary and secondary aptamers, as well as compositions comprising such primary and secondary aptamers, wherein analytes comprise small molecules that offer limited mutually non-competitive epitopes to antibodies, that is, with limited ability to measure in non-competitive sandwich assays using primary and secondary antibodies or primary and secondary aptamers.

Methods of detecting a target in a sample are provided. Kits for performing the methods described herein are also provided.

Methods of detecting a target in a sample are provided. Kits for performing the methods described herein are also provided.

This disclosure relates to the field of mass spectrometry analysis. In some embodiments, the disclosure relates to compositions and methods for detecting and quantifying proteins in the AKT-mTOR pathway by immunoprecipitation enrichment followed by mass spectrometry analysis.

The present invention provides compositions, kits, and methods for the detection of host cell proteins (HCPs) in biological samples. In some embodiments, the present invention utilizes immunization of aves hosts with proteins derived from non-aves host cells to produce aves antibodies specific for non-aves HCPs.

The present invention provides compositions, kits, and methods for the detection of host cell proteins (HCPs) in biological samples. In some embodiments, the present invention utilizes immunization of aves hosts with proteins derived from non-aves host cells to produce aves antibodies specific for non-aves HCPs.