A technology to determine an appropriate detection region in automatic determination of an immunochromatographic assay and an immunochromatographic assay interpretation device wherein a liquid sample including a detectable substance spreads into a region for detection via a test strip, and a negative or positive determination is made from the coloration state of the detection region, wherein a measuring unit acquires coloration index data for a range including at least a portion of a sample developing detection region for indicating that a sample has reached the detection region downstream from the detection region in the test strip, and a processing unit specifies the detection region from the coloration index data, the detection region in a predetermined inter-region distance upstream from the detection region of the coloration index data, and interprets negative or positive based on the coloration state of the detection region.

A vehicle control system with impaired driver prevention and biometric detection includes a vehicle with an ignition interlock circuit and one or more vehicle electrical systems, a detection device with an internal sensor system, and a mobile device having and one or more biometric sensors. The system operates by initiating a vehicle start command via the mobile device, prompting a user for a scan, receiving the scan from the detection device, whereby the scan includes at least a blood alcohol content percentage of the user, acquiring a sensed biometric image via the biometric sensors of the mobile device, and comparing the sensed biometric image to one or more stored biometric images. If the biometric image does not match a stored biometric image, or if the scan includes a blood alcohol content above a threshold value, then the vehicle start command is canceled.

The present invention has an object of providing: an immunochromatographic test strip, which controls the development of a specimen on the immunochromatographic test strip and appropriately controls a treatment with an acid reagent and a nitrite, and a neutralizing reagent; and an immunochromatography method using the test strip. The immunochromatographic test strip for extracting and measuring a sugar chain antigen in a specimen, comprising:

a sample pad to which the specimen mixed with a nitrite or an acid solution is added;
a label region comprising a labeled antibody obtained by labeling an antibody against the sugar chain antigen; and a detection region on which an antibody against the sugar chain antigen is immobilized,
wherein an antibody-sugar chain antigen-labeled antibody complex is formed in the detection region to measure the sugar chain antigen, and wherein the immunochromatographic test strip has a region impregnated with a neutralizing reagent upstream of the label region, and further has, upstream of the region impregnated with the neutralizing reagent, a region impregnated with a solid acid reagent when the specimen mixed with the nitrite is used, or a region impregnated with the nitrite when the specimen mixed with the acid solution is used,
wherein a hydrophobic material is used for the region impregnated with the nitrite or the solid acid reagent.

A saliva sensing device for detecting oral health and/or overall health includes a chemically treated test strip biomarker targeted to identify bacteria in the saliva. A breathalyzer for detecting bacteria in the mouth of a user includes a housing, a sensor, and an analyzer. The sensor is coupled to the housing and extends outwardly therefrom for placing in the mouth of user and for collecting a saliva sample. The analyzer is for testing saliva that contacts the sensor.

A method of diagnosing the risk that a subject has or has an increased risk of developing overactive bladder syndrome is described. The method includes detecting the levels of choline and/or acetylcholine in a biological sample from the subject, comparing the detected levels to reference values, and characterizing the subject as having an increased risk of having or developing overactive bladder syndrome if the choline and/or acetylcholine values are higher than the reference values. Kits for determining choline and/or acetylcholine levels in a subject, and methods of treating a subject for overactive bladder syndrome are also described.

Disclosed are a one-pot biosensor and an immunoassay method using the same. The one-pot biosensor includes a photocatalyst substrate deposited with metal nanoparticles; and a reaction pad which is disposed on an upper surface of the photocatalyst substrate and includes a first binding material-fluorescent material complex specifically binding to a molecule to be detected, and the immunoassay method using the same. The one-pot biosensor may detect a target by once solution injection and has a size enough to be portable. Accordingly, since the one-pot biosensor can detect the target by only once solution injection without a washing step, because of a sensor platform capable of being easily used by an individual other than a diagnostic expert, it is predicted to be positioned as a means capable of confirming the health condition of the individual without seeing the doctor, such as a pregnancy diagnostic kit which has been currently commercialized.

A diagnostic testing device along with a method of manufacturing the diagnostic testing device is provided. The diagnostic testing device includes a sheet of foam; a top web including a recess shaped and sized for a lateral flow matrix; a bottom web attachable to the top web; and a sealing layer allocated on the top web configured to prevent contamination of the lateral flow matrix; wherein the lateral flow matrix includes a sample port. The method of manufacturing includes a series of die cutting mechanisms applied to various webs of material unified via a lamination process.

An exhaled breath condensate (EBC) collection device comprising two adjacent parts foldable together and apart from each other, a condensation zone to condense exhaled breath from a subject, the condensation zone being a third part provided between the two adjacent parts and connectable to cooling means arranged in each of the two parts being in cooling relationship with the condensation zone. There is also provided a method for detecting antigens or antibodies to the antigens, the method comprising collecting EBC from a subject using the EBC collection device, performing immunochromatographic testing (ICT) of the collected exhaled breath condensate, and interpreting the immunochromatographic testing results as follows: if the immunochromatographic testing results are positive, the antigens or antibodies to the antigens are detected, or if the immunochromatographic testing results are negative, the antigens or antibodies to the antigens are not detected.

Devices and methods to perform a competitive immunoassay are disclosed. In some embodiments, the competitive immunoassay is for the detection of tacrolimus. The devices comprise a plurality of layers optionally made of cellulose-based material, wherein the plurality of layers comprises at least one read-out layer displaying a colorimetric readout of the device to indicate the result of the test to the user.

The present invention provides a lateral flow test device for detecting a coronavirus antibody by immunoassay; the test device includes three lateral flow test strips; a first test strip is directed to the antibody detection of a N full-length protein and/or an S full-length protein antigens/antigen; and a second test strip is directed to the antibody detection of an S-RBD-site protein antigen, and both of the first strip and the second strip are combined to detect novel coronavirus IgG and IgM antibodies, which can practically reduce the possibility of missing detection and wrong detection; further, a third test strip is directed to the detection of a neutralizing antibody at an S-RBD site to rapidly detect the neutralizing antibody having protection effect, thus further helping the prevention of missing detection and helping patients to perform self-detection and judge the situations of recovery, or vaccine immunity.