The present invention provides an anti-human hemoglobin monoclonal antibody or an antibody kit for specifically detecting and measuring a hemoglobin-haptoglobin complex in a sample with ease, and an insoluble carrier particle to which the monoclonal antibody is immobilized, and a measurement reagent and a measurement method for specifically detecting and measuring a hemoglobin-haptoglobin complex in a sample using the same. The anti-human hemoglobin monoclonal antibody of the present invention does not react to free hemoglobin or free haptoglobin which is not formed in a complex, but specifically reacts to a hemoglobin-haptoglobin complex when the antibody is immobilized to an insoluble carrier particle and used.

The present invention provides an anti-human hemoglobin monoclonal antibody or an antibody kit for specifically detecting and measuring a hemoglobin-haptoglobin complex in a sample with ease, and an insoluble carrier particle to which the monoclonal antibody is immobilized, and a measurement reagent and a measurement method for specifically detecting and measuring a hemoglobin-haptoglobin complex in a sample using the same. The anti-human hemoglobin monoclonal antibody of the present invention does not react to free hemoglobin or free haptoglobin which is not formed in a complex, but specifically reacts to a hemoglobin-haptoglobin complex when the antibody is immobilized to an insoluble carrier particle and used.

Provided are a latex agglutination reagent containing polyoxazoline that is preferable for use in a latex agglutination reaction and has a high sensitization action of sensitivity while a non-specific reaction can be suppressed, and a method for measuring a target substance in a specimen by a latex agglutination method. A latex agglutination reagent used in a method for measuring a target substance in a specimen by a latex agglutination method using an insoluble carrier that carries an antibody or an antigen contains polyoxazoline having a repeating unit represented by the following General Formula

##STR00001##

(in Formula (1), R represents a hydrogen atom or a methyl group, an ethyl group, or a propyl group, and n is an integer of 100 or greater).

Provided are a latex agglutination reagent containing polyoxazoline that is preferable for use in a latex agglutination reaction and has a high sensitization action of sensitivity while a non-specific reaction can be suppressed, and a method for measuring a target substance in a specimen by a latex agglutination method. A latex agglutination reagent used in a method for measuring a target substance in a specimen by a latex agglutination method using an insoluble carrier that carries an antibody or an antigen contains polyoxazoline having a repeating unit represented by the following General Formula

##STR00001##

(in Formula (1), R represents a hydrogen atom or a methyl group, an ethyl group, or a propyl group, and n is an integer of 100 or greater).

An object of the present invention is to provide a solid phase carrier for carrying a bioactive substance, which achieves both the high non-specific adsorption inhibitory effect and high ligand binding amount. Another object of the present invention is to provide a kit for measuring a measurement target substance in a biosample, which includes the solid phase carrier. According to an embodiment of the present invention, a solid phase carrier for carrying a bioactive substance is provided which includes a copolymer present on a surface of the solid phase. The copolymer has a structural unit in which a hydrophilic group and a bioactive substance-reactive group are bonded to an acidic amino acid-derived group through a predetermined linking group.

An object of the present invention is to provide a solid phase carrier for carrying a bioactive substance, which achieves both the high non-specific adsorption inhibitory effect and high ligand binding amount. Another object of the present invention is to provide a kit for measuring a measurement target substance in a biosample, which includes the solid phase carrier. According to an embodiment of the present invention, a solid phase carrier for carrying a bioactive substance is provided which includes a copolymer present on a surface of the solid phase. The copolymer has a structural unit in which a hydrophilic group and a bioactive substance-reactive group are bonded to an acidic amino acid-derived group through a predetermined linking group.

Provided herein are multiplexible particle systems and related methods of making and using the multiplexible particle systems. A plurality of monodisperse polymer particle populations are provided, wherein each population has a unique electrical parameter for multiplexed detection by flow through a spatially confined electric field, and the distribution of the electrical parameter within each population is sufficiently narrow for reliable multiplex detection. The density difference between populations may be relatively uniform, such as within 30%, including within 30% of a suspending solution density for when the particles are flowed through a confined electric field and detected in a multiplex manner by a change in the electric parameter measured by a counting device. Relatively uniform density of particles is important for ensuring minimal settling while the plurality of particle populations flow together under a single flow regime. The multiplexible particle systems are used in applications including multiplex detection or quantification, electrically barcoding, sorting, and counting.

Provided herein are multiplexible particle systems and related methods of making and using the multiplexible particle systems. A plurality of monodisperse polymer particle populations are provided, wherein each population has a unique electrical parameter for multiplexed detection by flow through a spatially confined electric field, and the distribution of the electrical parameter within each population is sufficiently narrow for reliable multiplex detection. The density difference between populations may be relatively uniform, such as within 30%, including within 30% of a suspending solution density for when the particles are flowed through a confined electric field and detected in a multiplex manner by a change in the electric parameter measured by a counting device. Relatively uniform density of particles is important for ensuring minimal settling while the plurality of particle populations flow together under a single flow regime. The multiplexible particle systems are used in applications including multiplex detection or quantification, electrically barcoding, sorting, and counting.

The present invention relates to latex particles for measurement reagents, said latex particles having a coefficient of variation in particle diameters of 10% or less and an average particle diameter of 250 to 1000 nm. Each of the latex particles contains 20% by weight or more of a compound having a refractive index of 1.60 or more. The depth of a supernatant is 5 mm or less when a liquid dispersion having a solid matter concentration of 1% by weight, which is prepared by dispersing the latex particles in ultrapure water, is placed in a tubular 10-ml measuring cylinder having a body inner diameter of 10.8 mm and is then allowed to leave for 10 days. According to the particles, a measurement of a measurement sample can be performed at a high sensitivity even when the concentration of a substance to be tested in the measurement sample is low.

The present invention relates to latex particles for measurement reagents, said latex particles having a coefficient of variation in particle diameters of 10% or less and an average particle diameter of 250 to 1000 nm. Each of the latex particles contains 20% by weight or more of a compound having a refractive index of 1.60 or more. The depth of a supernatant is 5 mm or less when a liquid dispersion having a solid matter concentration of 1% by weight, which is prepared by dispersing the latex particles in ultrapure water, is placed in a tubular 10-ml measuring cylinder having a body inner diameter of 10.8 mm and is then allowed to leave for 10 days. According to the particles, a measurement of a measurement sample can be performed at a high sensitivity even when the concentration of a substance to be tested in the measurement sample is low.