A photocleavable heterobifunctional linker can include a structure of Formula (A) wherein coumarin is any coumarin or coumarin derivative; R, R.sup.9, and R.sup.10 are each independently a chemical moiety; R.sup.1 is a hydrogen, protecting group, leaving group, substrate, or capture entity; R.sup.2 is a hydrogen, hydroxyl, halide, alkoxy, anhydride, amino, protecting group, leaving group, substrate, or capture entity; L.sup.1 is a sub-linker; and L.sup.2 is a sub-linker. A capture device can include the photocleavable bifunctional linker having a structure of Formula (A) as provide herein, wherein R.sup.1 is a substrate. A method of capturing a target substance can include: providing the capture device having the photocleavable bifunctional linker with the structure of Formula (A) and contacting a target substance to the capture moiety such that the target substance is captured. Irradiating the linker with light can cleave the linker, thereby releasing the target substance from the substrate.

Provided is a solid phase carrier which has high water dispersibility, allows facilitated binding of a ligand to a reactive functional group, and exhibits suppressed non-specific adsorption, and with which, in the case of using the solid phase carrier by having a ligand bound thereto, for example, detection of a target substance can be carried out with high sensitivity and low noise. Disclosed is a solid phase carrier having bound thereto a polymer including a structural unit represented by the following Formula (1) and a structural unit represented by the following Formula (2):

##STR00001##

wherein in Formula (1), R.sup.1 represents a hydrogen atom or a methyl group; and R.sup.2 represents an organic group having a zwitterionic structure,

##STR00002## in Formula (2), R.sup.3 represents a hydrogen atom or a methyl group R.sup.4 represents —(C═O)—O—*, —(C═O)—NR.sup.6—* (wherein R.sup.6 represents a hydrogen atom or a methyl group; and the symbol * represents a position of bonding to R.sup.5 in Formula (2)), or a phenylene group; in a case in which R.sup.4 represents —(C═O)—O—*, R.sup.5 represents a hydrogen atom, or an organic group having a reactive functional group, and in a case in which R.sup.4 represents —(C═O)—NR.sup.6—* or a phenylene group, R.sup.5 represents an organic group having a reactive functional group, provided that R.sup.5 is not an organic group having a zwitterionic structure.

Provided is a solid phase carrier which has high water dispersibility, allows facilitated binding of a ligand to a reactive functional group, and exhibits suppressed non-specific adsorption, and with which, in the case of using the solid phase carrier by having a ligand bound thereto, for example, detection of a target substance can be carried out with high sensitivity and low noise. Disclosed is a solid phase carrier having bound thereto a polymer including a structural unit represented by the following Formula (1) and a structural unit represented by the following Formula (2):

##STR00001##

wherein in Formula (1), R.sup.1 represents a hydrogen atom or a methyl group; and R.sup.2 represents an organic group having a zwitterionic structure,

##STR00002## in Formula (2), R.sup.3 represents a hydrogen atom or a methyl group R.sup.4 represents —(C═O)—O—*, —(C═O)—NR.sup.6—* (wherein R.sup.6 represents a hydrogen atom or a methyl group; and the symbol * represents a position of bonding to R.sup.5 in Formula (2)), or a phenylene group; in a case in which R.sup.4 represents —(C═O)—O—*, R.sup.5 represents a hydrogen atom, or an organic group having a reactive functional group, and in a case in which R.sup.4 represents —(C═O)—NR.sup.6—* or a phenylene group, R.sup.5 represents an organic group having a reactive functional group, provided that R.sup.5 is not an organic group having a zwitterionic structure.

A method of fabricating a substrate for analysis fixes antibodies that specifically react with specific antigens included in detection target substances to the substrate for analysis. The method subjects the substrate for analysis to immersion treatment with a predetermined treatment solution so as to form antibody aggregations in which the antibodies are aggregated at boundary regions between recesses and convex portions on the substrate for analysis. The method causes the antigens and the antibodies to react with each other so as to capture the detection target substances on the substrate for analysis by the antibody aggregations.

A method of fabricating a substrate for analysis fixes antibodies that specifically react with specific antigens included in detection target substances to the substrate for analysis. The method subjects the substrate for analysis to immersion treatment with a predetermined treatment solution so as to form antibody aggregations in which the antibodies are aggregated at boundary regions between recesses and convex portions on the substrate for analysis. The method causes the antigens and the antibodies to react with each other so as to capture the detection target substances on the substrate for analysis by the antibody aggregations.

This document relates to methods and materials for assessing and/or treating mammals (e.g., humans) having, or suspected of having, cancer. For example, methods and materials for identifying a mammal as having cancer are provided. For example, microfluidic devices that can be used to detect one or more target polypeptides (e.g., cancer-specific polypeptides) in a fluid sample obtained from a mammal (e.g., a mammal suspected of having cancer) are provided.

Methods of determining infection type are disclosed. In one embodiment, the method comprises measuring the amount of TRAIL and/or IP10 no more than two days from symptom onset.

Methods of determining infection type are disclosed. In one embodiment, the method comprises measuring the amount of TRAIL and/or IP10 no more than two days from symptom onset.

The present disclosure relates to a kit for detecting dust mite component-specific antibodies, and belongs to the technical field of antibody detection kits. The kit comprises a biotin-polystreptavidin-biotin-dust mite antigen-coated nitrocellulose (NC) membrane, a washing solution, an alkaline phosphatase (ALP)-labeled secondary antibody solution for dust mite component-specific antibodies, and a substrate solution. The kit may be used in cooperation with a fully automated instrument.

The present disclosure relates to a kit for detecting dust mite component-specific antibodies, and belongs to the technical field of antibody detection kits. The kit comprises a biotin-polystreptavidin-biotin-dust mite antigen-coated nitrocellulose (NC) membrane, a washing solution, an alkaline phosphatase (ALP)-labeled secondary antibody solution for dust mite component-specific antibodies, and a substrate solution. The kit may be used in cooperation with a fully automated instrument.