The disclosure provides a powder form and a hydrogel composition for alleviating degenerative joint and tendon tear. The powder form consists essentially of a 120-380 parts by weight biodegradable copolymer; 15-75 parts by weight urea; and 100 parts by weight platelet-rich plasma; wherein the biodegradable copolymer has a structure of formula (I) or formula (II):

##STR00001##

wherein A comprises a hydrophilic polyethylene glycol polymer; B comprises a hydrophobic polyester polymer; BOX is bifunctional group monomer of 2, 2-bis(2-oxazoline) used for coupling the blocks AB or BAB; and n is an integer greater than or equal to 0.

Techniques for opening a target tissue using nanodroplets are provided. An exemplary method can include targeting a region of tissue for opening, delivering a plurality of nanodroplets to the region, and applying an ultrasound beam at the region such that the nanodroplets cavitate, or convert to microbubbles that cavitate, thereby causing the target tissue to open.

A system for treating infected tissue includes an applicator device. The applicator device has an electrode pair spaced apart and is positioned against infected tissue. An electrical power supply to generate a pulsed electric field across the electrode pair, resulting in the application of a pulsed electric field through the infected tissue. The pulsed electric field has a field strength within a range that when the pulsed electric field is applied, pores are formed in membranes of cells of the infected tissue. A method for treating infected tissue is disclosed. The method includes positioning an electrode pair against the infected tissue; applying a pulsed electric field across the electrode pair. The pulsed electric field has a field strength that when applied, pores are formed in membranes of cells of the infected tissue; and applying an antimicrobial agent into or onto the infected tissue when the pulsed electric field is applied.

Disclosed herein are glycan-coated RBD immunogens, engineered nanoparticle vaccines comprising glycan-coated RBD immunogens and methods of use thereof for SARS-COV-2 vaccines.

A method for loading immunocompetent cells with nanoparticles and/or a cytotoxic substance is for use in the theranostic treatment of patient-specific genetically modified cells. The method includes the steps: (a) providing immunocompetent cells; and (b) loading the immunocompetent cells with the nanoparticles and/or the cytotoxic substance, wherein the nanoparticles and/or the cytotoxic substance are nuclides for radiotherapy. The loading step includes (i) applying the nanoparticles and/or the cytotoxic substance to the immunocompetent cells or mixing the nanoparticles and/or the cytotoxic substance with the immunocompetent cells in a liquid carrier; and (ii) poration of the immunocompetent cells in order to increase the permeability of the cell membranes of the immunocompetent cells for the nanoparticles and/or the cytotoxic substance and thereby loading the immunocompetent cells with the nanoparticles and/or the cytotoxic substance.

A method for porating cells for loading cells with a substance, preferably nuclides for radiotherapy, performs porating in a porating device with cells to be porated in a liquid with the substance. The cell membranes are permeabilized by sonoporation or laser poration in a reactor under real-time-monitoring. Moreover, a device for porating cells, in particular by sonoporation or laser porating, and for loading cells, in particular immunocompetent cells, in particular ?? T cells, includes inlet and outlet openings for a cell solution with cells to be porated and a substance to be taken up by the cells as a load and preferably microbubbles. A poration arrangement extending between the openings has a collector at the inlet opening for collecting cell solution and a reactor in flow connection with the collector. Inside the reactor the cell membranes may be porated and the cells may take up the load.

Disclosed herein is a vaccine comprising an antigen and one or more bimolecular adjuvant. Also disclosed herein are methods for increasing an immune response in a subject. The methods may comprise administering the vaccine to the subject in need thereof.

Provided among other things is a method of treating a diseased tissue that is a cancer tissue, virally infected tissue, or a tissue infected with an obligate intracellular parasite, in a large animal comprising: (a) administering a therapeutic agent effective for treating the disease to the animal; and (2) using over a period of time one or more phased arrays of antennas to direct pulses of radio frequency or microwave radiation in permeability-enhancing amount to the affected tissue, wherein the pulsed radiation is effective to promote the activity of the therapeutic agent against cells of the cancerous tissue or against the activity of the pathogen.

This invention provides methods and systems for achieving high-specificity killing of targeted cells using Magneto-Electric Nano-Particles (MENPs) and functional or diagnostic imaging that detects changes at the cellular level. Embodiments comprise injecting into a patient's body manufactured MENPs that have a higher tendency to accumulate near or attach to targeted cells through one or more physical forces and/or biological mechanisms; and applying a magnetic field to the MENPs to generate an action that is sufficient to cause death of the targeted cells, and using an imaging apparatus to image or detect a specific property of the MENPs or changes in a property of the MENPs due to the coupling of the MENPs with their surrounding environment.

A combination of low frequency high amplitude sonic frequency vibrations and high frequency low intensity ultrasonic pressure waves are applied to cosmetic compounds and to the skin to promote improved penetration of the cosmetic compounds into the epidermis. The cosmetic applicator device includes means for generating both sonic frequency vibrations and ultrasonic pressure waves adopted to deliver cosmetic compounds into the epidermis safely without significant temperature rise in the skin. Various removable applicator and skin cleaning attachments are also disclosed, including some with ultrasound waveguide.