The present invention relates to a platelet testing device using blockage phenomenon, comprising: a sample chamber containing blood sample; a microfluidic tube which is in fluid communication with the sample chamber and through which the blood sample flows; and a microbead packing arranged on a flow path of the blood sample of the microfluidic tube; wherein the microbead packing comprises: a packing pipe which constitutes a part of the flow path of the blood sample; and a plurality of microbeads contained in the packing pipe and arranged to be in close contact with each other so as to form voids between the microbeads, whereby function of the platelet is tested by blockage phenomenon of the voids due to the platelet in the blood sample which flows through the microfluidic tube from the sample chamber according to the present invention.

The current invention relates to the method and apparatus to magnetically separate biological entities with magnetic labels from a fluid sample. The claimed magnetic separation device removes biological entities with magnetic labels from its fluidic solution by using a soft-magnetic center pole with two soft-magnetic side poles. The claimed device further includes processes to dissociate entities conglomerate after magnetic separation.

The present invention relates to a method of using a microfluidic chip comprising introducing a gas into the microfluidic chip to replace the liquid that has been introduced into the microfluidic chip and forming a micro-reaction chamber in the form of a liquid-in-gas in the microfluidic chip. The present invention also relates to a method for obtaining assay data, a computer program product embodied in a computer-readable medium and a kit. The methods described in the present invention are easy to operate, low cost, versatile, enabling rapid exchange of fluids, achieving efficient separation and capture of single particles with high purity. In addition, the methods can avoid clogging the chip and facilitate recycling.

To provide a microchip that is easily handled.

Provided is a microchip having a plate shape and including: a sample liquid inlet into which a sample liquid is introduced; a main flow path through which the sample liquid introduced from the sample liquid inlet flows; and a sorting flow path into which a target sample is sorted from the sample liquid, in which the sample liquid inlet and a terminal end of the sorting flow path are formed on a same side surface. Furthermore, a sample sorting kit including the microchip is also provided. Moreover, a microparticle sorting device on which the microchip is mounted is also provided.

A method is for detecting a biomarker within a sample of blood. The method may include processing the sample of blood with a microfluidic blood plasma separator and a plasmonic array biosensor, and flowing the sample of blood over a sensing surface of the plasmonic array biosensor. The sensing surface of the plasmonic array biosensor may have an ssDNA aptamer against the biomarker. The method may further include binding the biomarker in the sample of blood to the ssDNA aptamer of the plasmonic array biosensor, and detecting the biomarker in the sample of blood based upon LSPR altering a reflected optical signal from the plasmonic array biosensor.

A microfluidic device comprising: an inlet section, for receiving a body fluid sample, the inlet section comprising an inlet port arranged to receive a supply of body fluid; a metering function configured to receive body fluid from the inlet section and comprising a first channel; and a sequent section configured to receive the body fluid from the metering function and comprising a second channel, wherein the first channel comprises a capillary stop valve configured to interrupt or reduce flow of the body fluid therethrough, and a means for visual inspection arranged adjacent to the capillary stop valve, wherein a geometry and/or dimension of the inlet port is configured such that when the supply of body fluid to the inlet port is removed, the Laplace pressure of a body fluid meniscus at the inlet port is higher than a threshold pressure of the capillary stop valve.

A drug screening platform simulating hyperthermic intraperitoneal chemotherapy including a dielectrophoresis system, a microfluidic chip and a heating system is disclosed. The dielectrophoresis system is used to provide a dielectrophoresis force. The microfluidic chip includes a cell culture array and observation module and a drug mixing module. The cell culture array and observation module are used to arrange the cells into a three-dimensional structure through the dielectrophoresis force to construct a three-dimensional tumor microenvironment. The drug mixing module is coupled to the cell culture array and observation module and used to automatically split and mix the inputted drugs and output the drug combinations into the cell culture array and observation module. The heating system is used for real-time temperature sensing and heating control of the drug combinations on the microfluidic chip to simulate high-temperature drug environment when performing hyperthermic intraperitoneal chemotherapy on the three-dimensional tumor microenvironment.

The present invention relates to a biosensor, including: a blood cell separation membrane which separates blood cells from blood and allows plasma components to pass through; a microfluid channel through which the plasma components that have passed through the blood cell separation membrane flow; a lower substrate which allows the plasma components that have passed through the blood cell separation membrane to flow along the microfluid channel; and a pillar which connects the blood cell separation membrane and the lower substrate, in which an electrode is disposed in the pillar, and the pillar pushes and lifts the blood cell separation membrane by a predetermined distance. The biosensor of the present invention allows plasma, which is difficult to pass through the blood cell separation membrane due to surface tension, to easily pass through.

A method herein to isolate exosomes includes providing a microfluidic device having a spiral-shaped channel in fluid communication with two inlet ports and at least two outlet ports. One of the two inlet ports is proximal to an inner wall of the spiral-shaped channel and the other is proximal to an outer wall thereof. At least one of the outlet ports is in fluid communication with a container for storing isolated exosomes. A blood sample and sheath fluid are introduced into the inlet ports proximal to the outer and inner walls, respectively, to form a diluted sample in the spiral-shaped channel and driven through for exosomes recovery in the container. The spiral-shaped channel in fluid communication with a first outlet port includes a first outlet channel connecting the spiral-shaped channel to the first outlet port and is longer than other outlet channels respectively connecting the spiral-shaped channel to the other outlet ports. A method of identifying diabetes mellitus is also disclosed herein.

Some embodiments of the technology disclosed herein relate to a system having a hydrodynamic separator element defining an element inlet and an element outlet. The element outlet has a first element outlet and a second element outlet. The hydrodynamic separator element has a plurality of curved microfluidic channels in fluid communication. Each of the plurality of microfluidic channels are arranged to operate in parallel. Each microfluidic channel defines a channel inlet downstream of the element inlet and a channel outlet having a first channel outlet upstream of the first element outlet and a second channel outlet upstream of the second element outlet. A flow characteristic sensor is in sensing communication with the separator element. A controller is in data communication with the flow characteristic sensor, where the controller is configured to provide a first alert upon the flow characteristic being outside a first threshold.