The disclosure provides nucleic acids, and variants and fragments thereof, derived from strains of Bacillus thuringiensis encoding variant polypeptides having increased pesticidal activity against insect pests, including Lepidoptera and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The present disclosure relates to methods of treating or preventing a biofilm-related infection and methods of preventing and treating biofilm formation on indwelling medical devices, implants, and non-medical surfaces comprising administering at least one soluble microbial protein that is encoded by an exopolysaccharide biosynthetic operon or functional gene cluster, wherein the protein comprises a glycosyl hydrolase domain. The present disclosure further provides particular soluble glycosyl hydrolases and compositions thereof.

Disclosed herein are antimicrobial compositions that are substantially odorless. The antimicrobial compositions can comprise, for example, a first essential oil and a second essential oil, wherein the composition is substantially odorless. In some examples, the composition can further comprise a third essential oil. The compositions described herein can, for example, reduce the population of microbes, such as Staphylococcus aureus or Escherichia coli, by 5 log or more. Also disclosed herein are beverages and food products including the compositions disclosed herein. Also disclosed herein is milk including the compositions disclosed herein. Also disclosed herein are methods for treating or preventing a microbial infection in a subject, the methods comprising administering to the subject an effective amount of any of the compositions disclosed herein. Also disclosed herein are methods for combating bovine mastitis, the methods comprising applying any of the compositions disclosed herein to cow udders.

Disclosed herein are antimicrobial compositions that are substantially odorless. The antimicrobial compositions can comprise, for example, a first essential oil and a second essential oil, wherein the composition is substantially odorless. In some examples, the composition can further comprise a third essential oil. The compositions described herein can, for example, reduce the population of microbes, such as Staphylococcus aureus or Escherichia coli, by 5 log or more. Also disclosed herein are beverages and food products including the compositions disclosed herein. Also disclosed herein is milk including the compositions disclosed herein. Also disclosed herein are methods for treating or preventing a microbial infection in a subject, the methods comprising administering to the subject an effective amount of any of the compositions disclosed herein. Also disclosed herein are methods for combating bovine mastitis, the methods comprising applying any of the compositions disclosed herein to cow udders.

The invention discloses a synergist for improving crop resistance and fertilizer absorption capacity. The synergist includes the following components by weight: 20-30 parts of an oyster shell powder, 20-30 parts of a water chestnut skin powder, 10-20 parts of a chinaberry bark powder, 10-20 parts of a magnetic material, 10-20 parts of an illite powder, 5-10 parts of a shiitake mushroom polysaccharide extract. The synergist can, on one hand, improve the resistance of crops, especially reducing the pests and diseases significantly, improving waterlogging and drought resistance; and, on the other hand, greatly reduce the use of fertilizers, especially nitrogen fertilizers, preventing soil compaction. It can also significantly increase the content of active polysaccharides, especially β-glucan in crops. In addition, the raw materials are readily available, the cost is low, and there are significant economic benefits.

The invention discloses a synergist for improving crop resistance and fertilizer absorption capacity. The synergist includes the following components by weight: 20-30 parts of an oyster shell powder, 20-30 parts of a water chestnut skin powder, 10-20 parts of a chinaberry bark powder, 10-20 parts of a magnetic material, 10-20 parts of an illite powder, 5-10 parts of a shiitake mushroom polysaccharide extract. The synergist can, on one hand, improve the resistance of crops, especially reducing the pests and diseases significantly, improving waterlogging and drought resistance; and, on the other hand, greatly reduce the use of fertilizers, especially nitrogen fertilizers, preventing soil compaction. It can also significantly increase the content of active polysaccharides, especially β-glucan in crops. In addition, the raw materials are readily available, the cost is low, and there are significant economic benefits.

The invention discloses a synergist for improving crop resistance and fertilizer absorption capacity. The synergist includes the following components by weight: 20-30 parts of an oyster shell powder, 20-30 parts of a water chestnut skin powder, 10-20 parts of a chinaberry bark powder, 10-20 parts of a magnetic material, 10-20 parts of an illite powder, 5-10 parts of a shiitake mushroom polysaccharide extract. The synergist can, on one hand, improve the resistance of crops, especially reducing the pests and diseases significantly, improving waterlogging and drought resistance; and, on the other hand, greatly reduce the use of fertilizers, especially nitrogen fertilizers, preventing soil compaction. It can also significantly increase the content of active polysaccharides, especially β-glucan in crops. In addition, the raw materials are readily available, the cost is low, and there are significant economic benefits.

A regulator for increasing plant β-glucan content includes the following components by weight: 30-50 parts of a magnetic material, 20-30 parts of an oyster shell powder, 20-30 parts of an illite powder, and 10-20 parts of a mushroom polysaccharide extract.

A regulator for increasing plant β-glucan content includes the following components by weight: 30-50 parts of a magnetic material, 20-30 parts of an oyster shell powder, 20-30 parts of an illite powder, and 10-20 parts of a mushroom polysaccharide extract.

A regulator for increasing plant β-glucan content includes the following components by weight: 30-50 parts of a magnetic material, 20-30 parts of an oyster shell powder, 20-30 parts of an illite powder, and 10-20 parts of a mushroom polysaccharide extract.