A biological toxicity test method for evaluating an ecological safety of an advanced oxidation process comprising the following steps: (1) collecting (preparing) a waste water to be determined; (2) collecting the waste water and a tap water after the advanced oxidation process treatment; (3) subjecting Koi (Cyprinus carpio haematopterus) to the water after treatment for exposure to poison; (4) Determining an anti-oxidation enzyme activity of a liver of the Koi after exposure; (5) Data analyzing. By comparing the changes of liver enzyme activities in different water, the present method evaluates the toxicity changes of micro-pollutant containing water before and after treatment, which fills in the gap of the ecological risk assessment for advanced oxidation technology.

A biological toxicity test method for evaluating an ecological safety of an advanced oxidation process comprising the following steps: (1) collecting (preparing) a waste water to be determined; (2) collecting the waste water and a tap water after the advanced oxidation process treatment; (3) subjecting Koi (Cyprinus carpio haematopterus) to the water after treatment for exposure to poison; (4) Determining an anti-oxidation enzyme activity of a liver of the Koi after exposure; (5) Data analyzing. By comparing the changes of liver enzyme activities in different water, the present method evaluates the toxicity changes of micro-pollutant containing water before and after treatment, which fills in the gap of the ecological risk assessment for advanced oxidation technology.

Disclosed are methods, systems, and devices for implementing nanoparticles to encapsulate biomolecules such as enzymes. In one aspect, a nanoparticle device includes a shell structure including an internal layer structured to enclose a hollow interior region and include one or more holes penetrating through the internal layer, and an external layer formed of a porous material around the internal layer; and an enzyme contained within the interior region of the shell structure, the enzyme having entered the shell structure through the one or more holes and incapable of passing through the external layer, in which the pores are of a size that prevents the enzyme to pass through the pores while permitting substances smaller than the pore size to pass through the pores.

Disclosed are methods, systems, and devices for implementing nanoparticles to encapsulate biomolecules such as enzymes. In one aspect, a nanoparticle device includes a shell structure including an internal layer structured to enclose a hollow interior region and include one or more holes penetrating through the internal layer, and an external layer formed of a porous material around the internal layer; and an enzyme contained within the interior region of the shell structure, the enzyme having entered the shell structure through the one or more holes and incapable of passing through the external layer, in which the pores are of a size that prevents the enzyme to pass through the pores while permitting substances smaller than the pore size to pass through the pores.

The present invention relates to a diagnostic strip for an enzymatic test for determining the amount of sarcosine and creatinine based on the formed hydrogen peroxide in a biological sample or in an environmental sample with visual or electronic evaluation of intensity of the colour product. The diagnostic strip consists of a pad to which zones of an absorbent matrix are applied to conduct the test, consisting of sample zone (V) at one end of the strip followed by one or two reaction zones (R1), (R2), where to (R1) zone is applied reagent containing sodium salt of 3-(N-ethyl-3-methylaniline) propanesulfonic acid and to (R2) zone is applied reagent containing peroxidase and 4-amino-2,3-dimethyl-1-phenyl-3-pyrazole. Reaction zones are followed by detection zone (D) and beeswax zone (Z), forming the other end of diagnostic strip.

The present invention relates to a diagnostic strip for an enzymatic test for determining the amount of sarcosine and creatinine based on the formed hydrogen peroxide in a biological sample or in an environmental sample with visual or electronic evaluation of intensity of the colour product. The diagnostic strip consists of a pad to which zones of an absorbent matrix are applied to conduct the test, consisting of sample zone (V) at one end of the strip followed by one or two reaction zones (R1), (R2), where to (R1) zone is applied reagent containing sodium salt of 3-(N-ethyl-3-methylaniline) propanesulfonic acid and to (R2) zone is applied reagent containing peroxidase and 4-amino-2,3-dimethyl-1-phenyl-3-pyrazole. Reaction zones are followed by detection zone (D) and beeswax zone (Z), forming the other end of diagnostic strip.

The present invention relates to a method for removing oxygen, used in an electrochemical biosensor, and to a measurement system and a method for electrochemically determining a concentration of an analyte using said method.

The present invention relates to a method for removing oxygen, used in an electrochemical biosensor, and to a measurement system and a method for electrochemically determining a concentration of an analyte using said method.

Sarcoidosis is a multisystem disease characterized by granulomatous inflammation in affected organs. The present invention discloses kits and a system for a blood test using mycobacterial catalase-peroxidase that has a high positive predictive value for confirming a diagnosis of sarcoidosis.

The present invention relates to a method for improving drug metabolism function of human stem cell-derived hepatocytes. More precisely, the human stem cell-derived hepatocytes are similar in cell morphology to human hepatocytes but display reduced expressions of drug or alcohol metabolism associated enzymes and antioxidant enzymes. So, the inventors co-cultured the hepatocytes differentiated from human stem cells with mouse hepatic stellate cells. As a result, it was confirmed that alcohol mediated toxicity was reduced so that liver cell damage or apoptosis level was reduced. In the meantime, the expressions of drug and alcohol metabolism associated enzymes and antioxidant enzymes were increased.