A Pseudomonas sp. ECO-1 strain was preserved at the China General Microbiological Culture Collection Center (CGMCC) on Mar. 31, 2017, with the preservation number of CGMCC No. 13960. The Pseudomonas sp. ECO-1 strain was separated from POPs (Persistent Organic Pollutants) polluted soil for the first time. The bifunctional enzyme preparation capable of efficiently degrading polychlorinated biphenyl and atrazine was prepared by utilizing the strain for the first time; especially, the bifunctional enzyme preparation has remarkable degradation activity on the polychlorinated biphenyl which is difficult to degrade under an aerobic condition, which is completely different from functions of existing known Pseudomonas sp. and enzyme preparations thereof.

Bacterial cultures are provided that comprise a modified Pseudomonas aeruginosa bacterium missing or deficient in two or more virulence factors. The two or more virulence factors can be selected from exotoxin A, hemolytic phospholipase C, phenazine-specific methyltransferase, alpha-1,3-rhamnosyltransferase, and 3-phosphoshikimate 1-carboxyvinyltransferase. Certain of the modified Pseudomonas aeruginosa bacteria are also missing or deficient in one or more alginate acetylation enzymes including the alginate Oacetyltransferases AlgI, AlgJ, AlgF, AlgX, and/or the C5-mannuronan epimerase AlgG. Methods of producing alginate are also provided along with compositions comprising alginate produced by the modified Pseudomonas aeruginosa bacteria.

The disclosure relates to isolated microorganismsincluding novel strains of the microorganismsmicrobial consortia, and agricultural compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial consortia, and agricultural compositions comprising the same, in methods for imparting beneficial properties to target plant species. In particular aspects, the disclosure provides methods of increasing desirable plant traits in agronomically important crop species.

Disclosed are a composition including a Pseudomonas sp. strain having excellent plastic degradation activity and the use thereof. The Pseudomonas sp. Strain may include Pseudomonas sp. JNU 01 (Accession No: KCTC 14861BP).

Provided are Pseudomonas protegens mutant strain Pf5-NiF, Pf5-retS, or Pf5-retS-NiF, and a screening method therefor and an application thereof. By means of Red/ET recombination and direct cloning technologies, the NiF nitrogen fixation gene island in the genome of Pseudomonas stutzeri DSM4166, taken as a whole, is cloned into the genome of Pseudomonas protegens Pf5, so as to heterologously express the same successfully to obtain a genetically engineered strain Pf5-NiF, thereby bringing a biological nitrogen fixation function to Pseudomonas protegens Pf5 which does not own a biological nitrogen fixation function. In addition, gene-directed markerless knockout of retS gene in the genome of Pseudomonas protegens Pf5 is performed to obtain a genetically engineered strain Pf5-retS. Thus, the expression levels of an antibiotic 2,4-diacetylphloroglucinol and red pigment are increased, and a mutant strain of Pseudomonas protegens Pf5 having a stronger bactericidal activity is obtained.

The invention relates to the field of bioengineering. In particular, the invention relates to a heparinase-producing Pseudomonas stutzeri strain and a heparinase derived therefrom. Furthermore, the invention relates to the preparation and use of the heparinase.

The disclosure discloses recombinant Pseudomonas plecoglossicida for producing L-xylose and application thereof, and belongs to the technical field of bioengineering. According to the disclosure, a synthesized 2-ketogluconate reductase gene and a 2,5-diketogluconate reductase gene derived from Corynebaterium ATCC 31090 and a pyruvate decarboxylase gene derived from Saccharomyces cerevisiae are successfully expressed in a host P. plecoglossicida by a double plasmid system, and an obtained genetically engineered strain is fermented for 56 h in a shake flask, where the yield of L-xylose reaches 16.2 g/L, and the transformation rate reaches 20.3%; the obtained genetically engineered strain is fermented for 48 h and 44 h in 3 L and 15 L fermentors, respectively, where the yields of L-xylose reach 37.6 g/L and 45.8 g/L, respectively, and the glucose transformation rates are 47.0% and 57.3%, respectively. The method has the advantages of low raw material cost, no pollution to the environment, simple operation, and important economic and social benefits.

A process includes growing NRB in a nitrate reducing bacteria media to form a NRB culture and combining the NRB culture with a concentrated nitrate solution to form a NRB composition.

Disclosed are solid forms of menaquinol and processes for obtaining them by chemical or enzymatic reduction of menaquinone. Said solid forms possess high stability to oxidation which allows effective use of menaquinol in the most common formulations wherein vitamin K2 is used.

Cleaning compositions having an amylase enzyme and a glycosyl hydrolase enzyme. Methods of making and using cleaning compositions having an amylase enzyme and a glycosyl hydrolase enzyme.