The invention relates to a plasmid, a DNA assembly method and its application recombinant strain. The plasmid has single adjacent Type IIP and Type IIS RE recognition sites. The plasmid combines the properties of Type IIP and Type IIS REs to achieve recursive cycling, SCAR-free and repeat sequence assembly.

A method is useful for the fermentative production of L-lysine using a C. glutamicum strain having a mutated Kup transporter.

An aspartokinase variant, a microorganism comprising the variant, and a method for producing an aspartate-derived L-amino acid or a homoserine derivative thereof using the microorganism.

An aspartokinase variant, a microorganism comprising the variant, and a method for producing an aspartate-derived L-amino acid or a homoserine derivative thereof using the microorganism.

The present disclosure provides compositions and methods for using modified hydrogenotrophic microorganisms capable of biologically utilizing or converting CO and/or CO.sub.2 gas, optionally in the presence of H.sub.2, into methionine.

The present disclosure provides compositions and methods for using modified hydrogenotrophic microorganisms capable of biologically utilizing or converting CO and/or CO.sub.2 gas, optionally in the presence of H.sub.2, into methionine.

The present disclosure relates to a microorganism producing O-acetylhomoserine with high efficiency and a method for producing O-acetylhomoserine and L-methionine using the microorganism. The present disclosure provides a microorganism producing O-acetylhomoserine having an enhanced activity of a protein which is predicted to export O-acetylhomoserine, and a method for producing O-acetylhomoserine and L-methionine using the microorganism.

A proteinic biomass preparation comprising a non-native organism of the Clostridia class, which organism expresses (i) a modified aspartate kinase; (ii) a modified homoserine dehydrogenase; (iii) a modified homoserine kinase; (iv) a modified anthranilate synthase; (v) a functional lycopene pathway and the genes crtY, crtW, and crtZ; and/or (vi) a functional oleic acid pathway and the four gene operon (pfaABCD). Methods of producing proteinic biomass preparations are also described.

The present invention relates to a compound of general formula I

##STR00001##

The present invention also relates to a method of producing N-acetyl homoserine and/or derivatives thereof, the method comprising contacting at least one recombinant cell in an aqueous medium with acetate wherein the recombinant cell comprises an increased activity relative to a wild type cell of (a) an enzyme E.sub.1, a homoserine dehydrogenase (EC1.1.1.3) and/or an enzyme E.sub.5, an aspartokinase (EC2.7.2.4); and (b) an enzyme E.sub.2, a homoserine O-acetyl transferase (EC2.3.1.31)
and the acetate is maintained at a concentration of at least about 0.001 g/L in the aqueous medium.

A method of producing 2,4-dihydroxybutyric acid (2,4-DHB) by a synthetic pathway that includes transforming malate into 4-phospho-malate using a malate kinase, then transforming 4-phospho-malate into malate-4-semialdehyde using a malate semialdehyde dehydrogenase, and then transforming malate-4-semialdehyde into 2,4-DHB using a DHB dehydrogenase.