The invention relates to novel maize plants, seeds and compositions, as well as improvements to maize plant breeding and methods for creating modifications in maize plant genomes.

The present invention relates to the use of trehalose, and/or a derivative thereof, as a supplement to a culture medium for culturing hybrid plant embryos in in vitro embryo rescue. The addition of trehalose, and/or a derivative thereof, to the culture medium significantly increased the survival rate of the hybrid plant embryos.

The present invention relates to a method for conducting high-throughput and directed mutagenesis for sugarcane resistance to glyphosate by plasma. The method is as follows: sugarcane embryonic calli are irradiated by a plasma instrument under a sterile condition for mutagenesis, wherein the mutagenesis power is 140˜200 W, the discharging distance is 35˜45 mm, the mutagenesis time is 110˜140 s and the protective gas is nitrogen; buffering culture, moderate/high concentration of glyphosate stress screening, differentiation into seedlings, glyphosate stress screening of bottle seedlings and stress screening via spraying glyphosate on the leave surfaces of potted plants are conducted for the treated calli. The present invention has the advantages of safe operation, simplicity, practicability, high handling capacity, low contamination, and due to implementation of directed stress screening, high screening efficiency, decreased subsequent screening workload and visual identification of resistant mutant strains.

The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.

The invention provides a callus induction medium for blueberry tissue culture, it takes WPM medium as basic medium, comprises: 0.5-5.0 mg/L CPPU and 0.1-0.4 mg/L 2-ip. The present invention also provides a callus culture method of blueberry, the step thereof comprises: inoculating the blueberry explant into the above callus induction medium to conduct induction culture, to form blueberry callus. The present invention also discloses the medium combination and culture method to culture the above blueberry callus to blueberry tissue culture plant. For the above medium and culture method, the differentiation effect is good, efficiency is high, and can conduct continuous differentiation, and the effect to multiple varieties are all better.

The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.

The invention discloses a medium combination for a rapid propagation medium for blueberry stein segment tissue, wherein the pre-culture medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising 0.1-0.5 mg/L IAA, 0.05-0.6 mg/L CPPU; the rapid culture medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising: 0.2-0.5 mg/L ZT; the seedling growth medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising: 0.1-2.0 mg/L 2-ip; and the rooting medium takes ½ MS medium as basis medium, comprising: 0.3-4.0 mg/L NAA. The invention further discloses a method of using the above medium combination to conduct rapid propagation of blueberry stein segment tissue, the propagation rate of this method is rapid.

The invention relates to novel maize plants, seeds and compositions, as well as improvements to maize plant breeding and methods for creating modifications in maize plant genomes.

The invention relates to novel maize plants, seeds and compositions, as well as improvements to maize plant breeding and methods for creating modifications in maize plant genomes.

The present disclosure provides a method for obtaining adventitious tetraploid buds of Blumea balsamifera, comprising the following steps: selecting a root segment of diploid B. balsamifera as an explant, culturing the root segment in a chromosome doubling inducing medium supplemented with 0.025-0.1 mg/L 1-naphthaleneacetic acid (NAA), 1.0-2.0 mg/L 6-benzylaminopurine (6-BA), and 90-150 mg/L colchicine, inducing explant cells, and simultaneously doubling chromosomes and differentiating the adventitious buds. The present disclosure fills the blank of using a root of B. balsamifera as the explant and increases effective explant sources during the propagation, proliferation and biotechnological breeding of B. balsamifera. More importantly, root cells of the B. balsamifera are directly differentiated into adventitious buds while chromosomes are doubled, and a callus formation process is not needed, so that the technical links are simplified and the variation of regeneration buds and the generation of chimeras are reduced.