According to the present invention, there are provided a chamber for transplantation which has a high durability, and in which an enclosed biological constituent can be maintained for a long period of time because an interior space thereof is efficiently secured; and a method for manufacturing the chamber for transplantation. The chamber for transplantation includes one or more membranes for immunoisolation at a boundary between an inside and an outside of the chamber for transplantation, in which all of the membranes for immunoisolation include a porous membrane containing a polymer, and a joint portion at which the porous membranes are directly fusion welded to each other is provided. The method for manufacturing a chamber for transplantation includes preparing one or more porous membranes containing a polymer selected from polysulfone and polyethersulfone, bringing one part of the porous membrane into direct contact with another part of the porous membrane, and performing a heat fusion welding of the two parts that are in direct contact with each other at a temperature which is a glass transition temperature of the polymer or higher and lower than a melting point of the polymer.

A system and method for maintaining the vitality of an organ through negative pressure ventilation and perfusion. The system includes fluidically coupled components: an organ enclosure, a diaphragm enclosure, an actuator/pump, a perfusion system, and a reservoir. The actuator can displace a precise amount of a working fluid that displaces that precise amount of a sterile support fluid. The sterile fluid travels between the diaphragm enclosure and the organ enclosure, thereby ventilating the organ within the organ chamber. The perfusion system circulates a perfusate through the organ.

An apparatus for perfusing an organ includes: an organ chamber configured to provide physical protection to an organ; at least one fluid flow loop from an outlet connection of the organ chamber, through an oxygenator, through a pump, and to an inlet connection of the organ chamber; and a container enclosing the components of the apparatus, wherein the apparatus is portable.

The invention relates to a method of preservation of nucleic acid sequences in histological tissues which comprises: treating a concentrated formaldehyde solution in water with basic ion-exchange resins; diluting the resulting acid-deprived formaldehyde solution with phosphate buffer pH 7.2-7.4 up to a concentration ranging between 2 and 4%; contacting the resulting acid-deprived formaldehyde solution obtained with the tissue samples; optionally embedding the samples fixed in paraffin.

Microbiota restoration therapy compositions and methods for manufacturing, processing, and/or delivering microbiota restoration therapy compositions are disclosed. An example method for manufacturing a microbiota restoration therapy composition may include collecting a human fecal sample and adding a diluent to the human fecal sample to form a diluted sample. The diluent may include a cryoprotectant. The method may also include mixing the diluted sample with a mixing apparatus and filtering the diluted sample. Filtering may form a filtrate. The method may also include transferring the filtrate to a sample bag and sealing the sample bag.

Microbiota restoration therapy compositions and methods for manufacturing, processing, and/or delivering microbiota restoration therapy compositions are disclosed. An example method for manufacturing a microbiota restoration therapy composition may include collecting a human fecal sample and adding a diluent to the human fecal sample to form a diluted sample. The diluent may include a cryoprotectant. The method may also include mixing the diluted sample with a mixing apparatus and filtering the diluted sample. Filtering may form a filtrate. The method may also include transferring the filtrate to a sample bag and sealing the sample bag.

An organ perfusion system comprises: a perfusion fluid circuit (16) arranged to circulate perfusion fluid through the organ; a surrogate organ (126) arranged to be connected into the circuit in place of the organ so that the circuit can circulate fluid through the surrogate organ; and organ sensing means arranged to distinguish between the presence of the organ in the circuit and the presence of the surrogate organ in the circuit. The sensing means may comprise one or more pressure sensors (136, 137, 138), or a flow meter (125). Further aspects relate to adjusting the content of at least one component, such as oxygen or a nutrient, in the perfusion fluid. Bubble detection means (113), and means (74) to measure the amount of fluid secreted by or leaked from the organ, may also be provided.

In a method of ventilating excised lungs, a ventilation gas is supplied to an airway of a lung and a vacuum is formed around the lung. A quality of the vacuum is varied between a lower level and a higher level to cause the lung to breathe, while the pressure of the ventilation gas supplied to the airway is regulated to maintain a positive airway pressure in the airway of the lung. The vacuum may be cyclically varied between the two vacuum levels. The levels may be maintained substantially constant over a period of time, or one or both of the lower and higher levels may be adjusted during ventilation. The lung may be placed in a sealed chamber, and a vacuum is formed in the chamber around the lung.

The disclosure provides for a container system and method for the cryopreservation and resuspension of a body fluid. The container system may include a cryopreservation container comprising the body fluid in a cryopreservation liquid, a resuspension container comprising a resuspension solution, a connection tube sterilely connecting the cryopreservation container and the resuspension container, and at least one shut-off element actively associated with the connection tube.

An organ preservation system having; an organ chamber with a perfusate reservoir, a pump arranged to circulate perfusate, from the reservoir and passes the perfusate through a dialysis filter, oxygenator, and temperature and pressure sensors prior to entering the chamber where an organ is perfused. The organ rests on the platform such that perfusate leaving the organ flows into the perfusate reservoir. The dialysis filter having permeable tubes which allow perfusate constituents to be exchanged with dialysate flowing through the dialysis filter. The dialysate pass through an ion exchange resin removing selected constituents or waste products from the dialysate by absorption by the ion exchange resin. Following waste absorption, the dialysate is recycled to the dialyzer to again remove waste. Removing waste products from the perfusate by dialysis followed by removal of the waste products from the dialysate with the exchange resin, enables dialysate reuse for extended duration.