A method for treating a perishable product is provided, the method comprising: determining a desired product treatment outcome selected from sanitization, protection, preservation, or enhancement of the perishable product; after harvesting, wet or dry washing the perishable product; directly or indirectly applying a substance to the surface of the perishable product using a carrier; and packaging the perishable product. A system for treating a perishable product with a substance treatment is provided, the system comprising: one or more devices for directed substance application to a surface of the perishable products prior to final packaging of the perishable product using a carrier; and wherein the one or more devices is an electrostatically charging device or a nebulizer. Embodiments of the invention which use multiple, sequenced, and engineered applications enhance the efficacy of sanitizers and other functional substances for increasing the quality, safety, and overall shelf life of perishable products.

Methods and compositions for inhibiting or dispersing biofilms produced by Listeria monocytogenes on plant matter are described. Embodiments include using formulations comprising active chemical constituents and aqueous extracts or sap from certain trees. Methods of using and producing aqueous formulations derived from hickory and maple wood are provided. Formulations and methods are provided for preventing and dispersing exopolysaccharide-rich listerial biofilms.

Methods and compositions for inhibiting or dispersing biofilms produced by Listeria monocytogenes on plant matter are described. Embodiments include using formulations comprising active chemical constituents and aqueous extracts or sap from certain trees. Methods of using and producing aqueous formulations derived from hickory and maple wood are provided. Formulations and methods are provided for preventing and dispersing exopolysaccharide-rich listerial biofilms.

A produce wash system, method, and short-term wash treatment are provided. For example, the produce wash system includes a produce line including a short-term wash device followed by a wash device, a short-term wash treatment that is applied by the short-term wash device to a product, wherein the short-term wash treatment is applied using a spray device that creates micrometer sized droplets, and wherein the short-term wash treatment remains on the product for a pretreatment time that lasts until the product reaches the wash device, and a wash treatment that is applied by the wash device to the product, wherein the wash treatment rinses the short-term wash treatment from the product defining the end of the pretreatment time. The pretreatment time is set at or below a damage threshold time beyond which the short-term wash treatment damages the product beyond a damage threshold.

A produce wash system, method, and short-term wash treatment are provided. For example, the produce wash system includes a produce line including a short-term wash device followed by a wash device, a short-term wash treatment that is applied by the short-term wash device to a product, wherein the short-term wash treatment is applied using a spray device that creates micrometer sized droplets, and wherein the short-term wash treatment remains on the product for a pretreatment time that lasts until the product reaches the wash device, and a wash treatment that is applied by the wash device to the product, wherein the wash treatment rinses the short-term wash treatment from the product defining the end of the pretreatment time. The pretreatment time is set at or below a damage threshold time beyond which the short-term wash treatment damages the product beyond a damage threshold.

The disclosure provides an antimicrobial mixture of at least two volatile compounds derived from essential oils, at least one of which is a bactericide selected from cinnamaldehyde, diacetyl, and acetic acid with another being a fungicide selected from allyl isothiocyanate, hexanal, thymol, and 2-nonane. The antimicrobial mixture inhibits both bacterial and fungal pathogens that negatively impact the shelf-life of perishable agricultural commodities, and at concentrations sufficiently low to avoid phytotoxic effects to agricultural commodities held in closed shipping and storage systems.

The disclosure provides an antimicrobial mixture of at least two volatile compounds derived from essential oils, at least one of which is a bactericide selected from cinnamaldehyde, diacetyl, and acetic acid with another being a fungicide selected from allyl isothiocyanate, hexanal, thymol, and 2-nonane. The antimicrobial mixture inhibits both bacterial and fungal pathogens that negatively impact the shelf-life of perishable agricultural commodities, and at concentrations sufficiently low to avoid phytotoxic effects to agricultural commodities held in closed shipping and storage systems.

A liquid nitrogen quick-freezing preservation method for fresh bamboo shoots. The method includes performing a coating treatment on cuts of fresh bamboo shoots, which reduces the moisture loss through the cuts, slows down the degree of lignification of the bamboo shoots, mitigates color changes at the cuts, inhibits excessively fast reproduction of microorganisms at the cuts and prevents invasion of microorganisms from the cuts, so that a decrease in nutritional value is reduced, the deterioration of the appearance quality of the bamboo shoots is mitigated, and the nutritional value and commodity value of the bamboo shoots are maintained. The method further includes an intermittent ultrasonic treatment on the fresh bamboo shoots, so that coating substances form films on the surfaces of the cuts more easily, preventing the fresh bamboo shoots from being eroded by microorganisms and preventing nutrient substances from being decomposed and utilized by the microorganisms.

A liquid nitrogen quick-freezing preservation method for fresh bamboo shoots. The method includes performing a coating treatment on cuts of fresh bamboo shoots, which reduces the moisture loss through the cuts, slows down the degree of lignification of the bamboo shoots, mitigates color changes at the cuts, inhibits excessively fast reproduction of microorganisms at the cuts and prevents invasion of microorganisms from the cuts, so that a decrease in nutritional value is reduced, the deterioration of the appearance quality of the bamboo shoots is mitigated, and the nutritional value and commodity value of the bamboo shoots are maintained. The method further includes an intermittent ultrasonic treatment on the fresh bamboo shoots, so that coating substances form films on the surfaces of the cuts more easily, preventing the fresh bamboo shoots from being eroded by microorganisms and preventing nutrient substances from being decomposed and utilized by the microorganisms.

Various embodiments of the present invention relate to, among other things, a nano carrier platform for generating enhanced engineered water nanostructures (iEWNS) encapsulating and delivering reactive oxygen species (ROS) and, in some instances, other active ingredients, methods for inactivating at least one of viruses, bacteria, bacterial spores, and fungi on a substrate by applying iEWNS to the substrate.