A shaker assembly and method, of which the shaker assembly includes a shaker tank, a mixing tank in fluid communication with the shaker tank and positioned adjacent thereto, an overflow weir positioned between and separating the shaker tank and the mixing tank, a first shaker positioned over the shaker tank, and a second shaker. The first and second shakers are configured to operate in parallel to partially separate a solid from a liquid of a drilling waste fluid. During normal operation, at least some of the liquid flows from the first and second shakers to the shaker tank, and from the shaker tank over the overflow weir and into the mixing tank.

Acoustic perfusion devices for separating biological cells from other material in a fluid mixture are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid mixture is being drawn.

A liquid refining apparatus is disclosed. The liquid refining apparatus includes a substrate, a loader which is formed on the substrate and configured to receive a first liquid, a filter which is configured to reduce a concentration of at least one substance contained in the first liquid to obtain a second liquid with a reduced concentration of the at least one substance, a reactor which is configured to mix the second liquid with a reactant for target substance detection to obtain a third liquid containing, among a plurality of substances contained in the second liquid, a first substance which undergoes a predetermined reaction with the reactant and a second substance which does not undergo the predetermined reaction with the reactant, and a separator which is configured to separate the first substance and the second substance.

The object of the invention is to perform, rapidly and at a low cost, a pretreatment of an analysis sample containing a turbid substance. Provided is an analysis sample pretreatment apparatus in which a clarified liquid is obtained by removing a turbid substance from an analysis sample. The analysis sample pretreatment apparatus includes a cell configured to store the analysis sample, and a cell holder in which at least a part of a housing is opened to mount the cell. The cell holder includes an ultrasonic wave transducer and an ultrasonic wave reflection plate that are disposed on facing plane pairs while sandwiching the cell mounted inside the cell holder. The cell includes a first opening unit from which the analysis sample flows in, a second opening unit from which the clarified liquid flows out, and a third opening unit from which the turbid substance is discharged. In a state where the cell is mounted in the cell holder, the first opening unit is provided at a position lower than an upper end of the ultrasonic wave transducer in a vertical direction, or at a position higher than a lower end of the ultrasonic wave transducer in the vertical direction.

Beads with functionalized material applied to them are exposed to an acoustic field to trap, retain or pass the beads. The beads may include or be free of ferro magnetic material. The beads may be biocompatible or biodegradable for a host. The size of the beads may vary over a range, and/or be heterogenous or homogenous. The composition of the beads may include high, neutral or low acoustic contrast material. The chemistry of the functionalized material may be compatible with existing processes. The acoustic field may be generated, for example, in an acoustic angled wave device or in an acoustic fluidized bed.

A fluidic device includes: a channel that extends along a first axis and through which a fluid flows; an ultrasonic transmission part that is disposed at the channel and transmits an ultrasonic wave into the channel along a second axis orthogonal to the first axis in response to an input of a drive signal; and a controller that controls the ultrasonic transmission part. The controller measures impedance of the ultrasonic transmission part at a time when the ultrasonic transmission part is driven while changing a drive frequency of the drive signal within a predetermined range, specifies a drive frequency at which the impedance is a local maximum and sets the drive frequency at which the impedance is a local maximum as a first drive frequency, and inputs the drive signal of the first drive frequency to the ultrasonic transmission part.

Systems and methods for cleansing blood are disclosed herein. The methods include acoustically separating undesirable particles bound to capture particles from formed elements of whole blood. After introducing the capture particles to whole blood containing undesirable particles, the whole blood and capture particles are flowed through a microfluidic separation channel. At least one bulk acoustic transducer is attached to the microfluidic separation channel. A standing acoustic wave, imparted on the channel and its contents by the bulk acoustic transducer, drives the formed elements and undesirable particles bound to capture particles to specific aggregation axes. After aggregating the particles, the formed elements exit the separation channel through a first outlet and are returned to the patient. The undesirable particles, bound to the capture particles, exit through a second outlet and can be discarded to saved for later study.

The present disclosure provides for acoustofluidic centrifuge systems that can enrich and separate nanoparticles disposed in a fluid, such as liquid droplets, in a fast and efficient manner. Exemplary systems include a sound wave generator, such as a pair of slanted interdigitated transducers, and a containment boundary, such as a PDMS ring. The sound wave generator can produce surface acoustic waves that are capable of driving droplets to spin in a manner that can separate different sized particles into groups. In some embodiments, the acoustofluidic centrifuge system can include a plurality of containment boundaries in fluid communication with each other, allowing particles to separate between the containment boundaries. Methods of operating such systems, including methods of isolating different exosome subpopulations, are also disclosed.

The current invention relates to the method and apparatus to magnetically separate biological entities with magnetic labels from a fluid sample. The claimed magnetic separation device removes biological entities with magnetic labels from its fluidic solution by using a soft-magnetic center pole with two soft-magnetic side poles. The claimed device further includes processes to dissociate entities conglomerate after magnetic separation.

Disclosed is a multiple bag system for fractionating blood, the system including a fluid collecting bag including at least one outlet port; at least first and second sampling bags, each including at least one inlet port and at least one outlet port; and a unit for transferring fluid from the fluid collecting bag to the sampling bags; wherein the unit for transferring fluid includes an acoustic sorter. Also disclosed is a method for fractionating blood into blood products.