An impeller, for example, a Rushton impeller for a bioreactor system is disclosed. The impeller includes a hub, optionaly including a slot, a plurality of blades, and one or more turbulators. The plurality of blades is disposed along a circumferential direction of the hub and spaced apart from each other. Each of the plurality of blades is coupled to at least a portion of a circumference and/or a top surface of the hub. Each blade of the plurality of blades includes a pressure face and a suction face. The one or more turbulators is disposed on at least a portion of the suction face, the pressure face, or both, of a blade of the plurality of blades.

An apparatus for nitrogenizing a beverage utilizing a container having a chamber with a plurality of baffles. The container also includes a nitrogen regulator in the form of a collapsible orifice at the base of the container. An agitator in the form of an impeller creates a shear on the beverage during delivery of a stream of nitrogen into the container.

The present set of embodiments relate to a bioproduction system, method, and apparatus for creating a scalable bioreactor system. Specifically, the present set of embodiments enable the determination of bioreaction performance characteristics of a commercial scale by matching operational parameters between a small test scale bioreaction to that of a commercial scale bioreaction. The system and methods do not rely on simply making bioreactor apparatuses across scales the same dimensionally which would not account for differences in fluid dynamic properties between very small to very large volumes, but requires tuning of a variety of systems (mixing assembly, sparger system, and headspace airflow system) in conjunction with one another to achieve predictive outcomes.

A bag assembly for use with a heat exchanger includes a flexible bag having of one or more sheets of polymeric material, the bag having a first end that bounds a first compartment and an opposing second end that bounds a second compartment, a support structure being disposed between the first compartment and the second compartment so that the first compartment is separated and isolated from the second compartment. A first inlet port, a first outlet port, and a first drain port are coupled with the flexible bag so as to communicate with the first compartment. A second inlet port, a second outlet port, and a second drain port are coupled with the flexible bag so as to communicate with the second compartment.

The present set of embodiments relate to a bioproduction system, method, and apparatus for creating a scalable bioreactor system. Specifically, the present set of embodiments enable the determination of bioreaction performance characteristics of a commercial scale by matching operational parameters between a small test scale bioreaction to that of a commercial scale bioreaction. The system and methods do not rely on simply making bioreactor apparatuses across scales the same dimensionally which would not account for differences in fluid dynamic properties between very small to very large volumes, but requires tuning of a variety of systems (mixing assembly, sparger system, and headspace airflow system) in conjunction with one another to achieve predictive outcomes.

A method and a system for sequestering carbon dioxide (CO.sub.2) while producing freshwater are provided. An exemplary method includes producing saline water from a saline aquifer, desalinating at least a portion of the saline water, producing freshwater and waste brine, mixing waste CO.sub.2 with the waste brine forming a brine/CO.sub.2 mixture, and injecting the brine/CO.sub.2 mixture into the saline aquifer.

A bag assembly for use with a heat exchanger includes a flexible bag having of one or more sheets of polymeric material, the bag having a first end that bounds a first compartment and an opposing second end that bounds a second compartment, a support structure being disposed between the first compartment and the second compartment so that the first compartment is separated and isolated from the second compartment. A first inlet port, a first outlet port, and a first drain port are coupled with the flexible bag so as to communicate with the first compartment. A second inlet port, a second outlet port, and a second drain port are coupled with the flexible bag so as to communicate with the second compartment.

An impeller, for example, a Rushton impeller for a bioreactor system is disclosed. The impeller includes a hub, optionally including a slot, a plurality of blades, and one or more turbulators. The plurality of blades is disposed along a circumferential direction of the hub and spaced apart from each other. Each of the plurality of blades is coupled to at least a portion of a circumference and/or a top surface of the hub. Each blade of the plurality of blades includes a pressure face and a suction face. The one or more turbulators is disposed on at least a portion of the suction face, the pressure face, or both, of a blade of the plurality of blades.

A fluid transfer assembly for single use bioreactors includes a fluid transfer housing that can be mounted to the impeller shaft using a bearing that places the fluid transfer assembly directly below the lowest impeller but allows the impeller shaft to spin inside independently of the fluid transfer assembly. A fluid conduit connects the fluid transfer housing to a port in the single use bag wall which allows fluids to be introduced into the sparger and which also helps prevent the fluid transfer assembly from rotating with the impeller shaft.

The invention relates to a stirred tank reactor for gas-liquid mass transfer in a slurry. The reactor includes a reactor tank (1) having a first volume (V.sub.1), a drive shaft (2) that extends vertically in the reactor tank, a motor (3) for rotating the drive shaft (2), a main impeller (4) which is a downward pumping axial flow impeller attached to the drive shaft (2) to create a main flow pattern in the reactor tank, and a gas inlet (5) arranged to supply gas into the reactor tank (1) to be dispersed to the liquid. The reactor includes a mechanical gas sparging apparatus (6) comprising a dispersion chamber (7) having a second volume (V.sub.2) which is substantially smaller than the first volume (V.sub.1) of the reactor tank (1), the dispersion chamber being arranged coaxial with the drive shaft (2), and the gas inlet (5) being arranged to feed gas into the dispersion chamber (7), and mixing means (8, 9, 10, 11, 12) arranged within the dispersion chamber (7) for mixing the gas into liquid by dispersing the gas to fine bubbles before the bubbles enter the main flow pattern. The mixing power per unit volume inside the dispersion chamber (7) is significantly larger than the mixing power elsewhere in the reactor.