An apparatus to simulate biocide performance in crude oil pipeline conditions is disclosed. The apparatus includes: a reactor to simulate a two-phase crude oil pipeline which includes a crude oil phase above a water phase. The reactor has an agitator to control a flow of the water phase in the reactor in response to a motor that drives an agitation rate of the agitator. A crude oil inlet supplies crude oil to the reactor for the crude oil phase. A water inlet supplies water to the reactor for the water phase. A control circuit is configured by code to control a proportion of the water to the crude oil supplied to the reactor and to control the motor to drive a desired agitation rate of the agitator. A biocide inlet supplies biocide to the reactor. A water sample outlet enables sampling of the water phase of the reactor.

An apparatus to simulate biocide performance in crude oil pipeline conditions is disclosed. The apparatus includes: a reactor to simulate a two-phase crude oil pipeline which includes a crude oil phase above a water phase. The reactor has an agitator to control a flow of the water phase in the reactor in response to a motor that drives an agitation rate of the agitator. A crude oil inlet supplies crude oil to the reactor for the crude oil phase. A water inlet supplies water to the reactor for the water phase. A control circuit is configured by code to control a proportion of the water to the crude oil supplied to the reactor and to control the motor to drive a desired agitation rate of the agitator. A biocide inlet supplies biocide to the reactor. A water sample outlet enables sampling of the water phase of the reactor.

An apparatus to simulate biocide performance in crude oil pipeline conditions is disclosed. The apparatus includes: a reactor to simulate a two-phase crude oil pipeline which includes a crude oil phase above a water phase. The reactor has an agitator to control a flow of the water phase in the reactor in response to a motor that drives an agitation rate of the agitator. A crude oil inlet supplies crude oil to the reactor for the crude oil phase. A water inlet supplies water to the reactor for the water phase. A control circuit is configured by code to control a proportion of the water to the crude oil supplied to the reactor and to control the motor to drive a desired agitation rate of the agitator. A biocide inlet supplies biocide to the reactor. A water sample outlet enables sampling of the water phase of the reactor.

The present invention provides a device and method for sampling from a cell culture bioreactor. The invention is useful where it is required to determine the cell count, provide a sample for off-line analyses or remove a sample while at the same time retain sterility of the sample for quality control (QC) assessment. The device of the invention allows for facile aseptic sampling at one or more instances in time during a cell expansion in a bioreactor.

Provided herein is a method for sterile fluid transfer. A first array of conduits may be disposed in a first compartment having a planar face with an array of cavities, each containing a cannular protrusion fluidly connected to a conduit. A second array of conduits may be disposed in a second compartment having its own planar face with an array of surface cannular protrusions, each fluidly connected to a conduit in the second array. The method may include sterilizing the lumens of each conduit-protrusion pair; snugly juxtaposing the two planar faces to form an array of steam-tight chambers; introducing a sterilizing gas into the chambers; expelling the sterilizing gas from the chambers; moving each cannular protrusion towards its facing surface protrusion to reversibly form a fluid connection therebetween, while the chamber remains externally sealed, and transferring a fluid across at least one fluidic connection.

An apparatus to simulate biocide performance in crude oil pipeline conditions is disclosed. The apparatus includes: a reactor to simulate a two-phase crude oil pipeline which includes a crude oil phase above a water phase. The reactor has an agitator to control a flow of the water phase in the reactor in response to a motor that drives an agitation rate of the agitator. A crude oil inlet supplies crude oil to the reactor for the crude oil phase. A water inlet supplies water to the reactor for the water phase. A control circuit is configured by code to control a proportion of the water to the crude oil supplied to the reactor and to control the motor to drive a desired agitation rate of the agitator. A biocide inlet supplies biocide to the reactor. A water sample outlet enables sampling of the water phase of the reactor.

The present invention provides a device and method for sampling from a cell culture bioreactor. The invention is useful where it is required to determine the cell count, provide a sample for off-line analyses or remove a sample while at the same time retain sterility of the sample for quality control (QC) assessment. The device of the invention allows for facile aseptic sampling at one or more instances in time during a cell expansion in a bioreactor.

This culture device comprises: a duct having an inner wall surface which faces a culture space, and a mounting surface which faces the portion to be mounted to of the inner surface, the mounting surface being mounted to the portion to be mounted to, and an air passage being formed with the inner wall surface; and a microbial light source provided within the passage. A protrusion which comes into close contact with the mounting surface, and which protrudes towards the mounting surface, is formed on the portion to be mounted to, or a protrusion which comes into close contact with the portion to be mounted to and which protrudes towards the portion to be mounted to is formed on the mounting surface.