The present invention relates to a method of separating cultured cells, which is used in a configuration including: a first accommodation space; a cell suspension which is present in a liquid in the first accommodation space in a state in which a plurality of cells is connected to one another; a filter container which is hermetically connected to the first accommodation space and mounted with a cell dividing member having one or more through holes having an average diameter of 5 μm to 300 μm; and a second accommodation space which is hermetically connected to the filter container, in which the cell suspension flows from the first accommodation space to the second accommodation space through the filter, such that the connection between the cells is passively and mechanically separated while the cells collide with a fixed resistance portion of the filter.

A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.

A method for producing a structure including, on a main surface of a substrate, at least one elongated cavity having openings at opposing ends. The method includes providing a substrate having a main surface. On the main surface, a first pair of features are formed that protrude perpendicularly from the main surface. The features have elongated sidewalls and a top surface, are parallel to one another, are separated by a gap having a width s1 and a bottom area, and have a width w1 and a height h1. At least the main surface of the substrate and the first pair of features are brought in contact with a liquid, suitable for making a contact angle of less than 90 with the material of the elongated sidewalls and subsequently the substrate is dried.

The present invention relates to a method of separating cultured cells, which is used in a configuration including: a first accommodation space; a cell suspension which is present in a liquid in the first accommodation space in a state in which a plurality of cells is connected to one another; a filter container which is hermetically connected to the first accommodation space and mounted with a cell dividing member having one or more through holes having an average diameter of 5 m to 300 m; and a second accommodation space which is hermetically connected to the filter container, in which the cell suspension flows from the first accommodation space to the second accommodation space through the filter, such that the connection between the cells is passively and mechanically separated while the cells collide with a fixed resistance portion of the filter.

A method for producing a structure including, on a main surface of a substrate, at least one elongated cavity having openings at opposing ends. The method includes providing a substrate having a main surface. On the main surface, a first pair of features are formed that protrude perpendicularly from the main surface. The features have elongated sidewalls and a top surface, are parallel to one another, are separated by a gap having a width s1 and a bottom area, and have a width w1 and a height h1. At least the main surface of the substrate and the first pair of features are brought in contact with a liquid, suitable for making a contact angle of less than 90 with the material of the elongated sidewalls and subsequently the substrate is dried.

A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.