Compositions are provided comprising recombinant variants of the human clotting Factor Xa. Such compositions include a wide variety of isoforms and post-translational modifications of FXa and are useful for treating subjects in need of hemostasis.

Provided is an assembled bioreactor chamber comprising an upper end portion, at least one intermediate portion and a lower end portion for forming a chamber of the reactor chamber. The upper end portion and the lower end portion are respectively provided with an interface connected with a culture medium line, and the upper end portion and the intermediate portion, and the intermediate portion and the lower end portion are detachably connected in a sealed manner through a connecting mechanism; when the number of the intermediate portions is ≥2, the adjacent intermediate portions are detachably connected in a sealed manner through a connecting mechanism; and the chamber also comprises a cell carrier for cell attachment growth.

Provided is an assembled bioreactor chamber comprising an upper end portion, at least one intermediate portion and a lower end portion for forming a chamber of the reactor chamber. The upper end portion and the lower end portion are respectively provided with an interface connected with a culture medium line, and the upper end portion and the intermediate portion, and the intermediate portion and the lower end portion are detachably connected in a sealed manner through a connecting mechanism; when the number of the intermediate portions is ≥2, the adjacent intermediate portions are detachably connected in a sealed manner through a connecting mechanism; and the chamber also comprises a cell carrier for cell attachment growth.

Disclosed herein is a bioreactor system that allows perfusive flow through a porous support medium enabling 3D growth of biological samples. In some embodiments, the system comprises a sample well filled with a three-dimensional (3D) cell growth medium. The system can further comprises a liquid medium reservoir fluidly connected to the sample well by a first filter material. The system can further comprises a medium collection chamber fluidly connected to the sample well by a second filter material. The system can further comprise an absorbant material that creates an osmotic pressure gradient to produce perfusive flow. In some embodiments, osmotic pressure draws fluid from the liquid medium reservoir, through the first filter material, into the sample well where it permeates the three-dimensional cell growth medium, through the second filter material, and finally into the medium collection chamber.

Disclosed herein is a bioreactor system that allows perfusive flow through a porous support medium enabling 3D growth of biological samples. In some embodiments, the system comprises a sample well filled with a three-dimensional (3D) cell growth medium. The system can further comprises a liquid medium reservoir fluidly connected to the sample well by a first filter material. The system can further comprises a medium collection chamber fluidly connected to the sample well by a second filter material. The system can further comprise an absorbant material that creates an osmotic pressure gradient to produce perfusive flow. In some embodiments, osmotic pressure draws fluid from the liquid medium reservoir, through the first filter material, into the sample well where it permeates the three-dimensional cell growth medium, through the second filter material, and finally into the medium collection chamber.

Disclosed herein is a bioreactor system that allows perfusive flow through a porous support medium enabling 3D growth of biological samples. In some embodiments, the system comprises a sample well filled with a three-dimensional (3D) cell growth medium. The system can further comprises a liquid medium reservoir fluidly connected to the sample well by a first filter material. The system can further comprises a medium collection chamber fluidly connected to the sample well by a second filter material. The system can further comprise an absorbant material that creates an osmotic pressure gradient to produce perfusive flow. In some embodiments, osmotic pressure draws fluid from the liquid medium reservoir, through the first filter material, into the sample well where it permeates the three-dimensional cell growth medium, through the second filter material, and finally into the medium collection chamber.

The present invention relates to a method and a device for fermentation based on a microbial asexual reproduction. The method comprises the steps of adding plural silica sands, a medium and a microorganism into a tank; and stirring for separating a cluster of the microorganism by a shear force generated from the plural silica sands and returning the microorganism to a logarithmic growth phase without undergoing a spore phase to increase a fermentation rate of the microorganism. The device comprises a tank, a speed control motor disposed outside the tank, a stirring component connected to the speed control motor, a refluxing mechanism for high pressure air and water connected to the tank, a heating unit disposed in the tank and a pumping motor connected to the tank.

When conducting an analysis of a sample using an X-ray fluorescence spectrometer, a user allows a sample holder to hold the sample such that the sample is sandwiched between a first film and a second film, and mounts the sample holder in this state to the spectrometer. When conducting an analysis of the sample using a Fourier transform infrared spectrometer, the user allows the sample holder to hold the sample such that the sample is not sandwiched between the first film and the second film, and mounts the sample holder in this state to the spectrometer.

Provided is an assembled bioreactor chamber comprising an upper end portion, at least one intermediate portion and a lower end portion for forming a chamber of the reactor chamber. The upper end portion and the lower end portion are respectively provided with an interface connected with a culture medium line, and the upper end portion and the intermediate portion, and the intermediate portion and the lower end portion are detachably connected in a sealed manner through a connecting mechanism; when the number of the intermediate portions is 2, the adjacent intermediate portions are detachably connected in a sealed manner through a connecting mechanism; and the chamber also comprises a cell carrier for cell attachment growth.

Provided is an assembled bioreactor chamber comprising an upper end portion, at least one intermediate portion and a lower end portion for forming a chamber of the reactor chamber. The upper end portion and the lower end portion are respectively provided with an interface connected with a culture medium line, and the upper end portion and the intermediate portion, and the intermediate portion and the lower end portion are detachably connected in a sealed manner through a connecting mechanism; when the number of the intermediate portions is 2, the adjacent intermediate portions are detachably connected in a sealed manner through a connecting mechanism; and the chamber also comprises a cell carrier for cell attachment growth.