The present disclosure generally relates to a pretreatment arrangement (100) for pretreatment of lignocellulosic biomass comprising a reactor vessel (101) extending along a longitudinal center line (102) and having an upstream inlet (103) for receiving biomass and a downstream outlet (104) for discharging biomass. The pretreatment arrangement (100) further comprising a scraping device (108) configured to scrape the interior walls (111) of the reactor vessel (101) and prevent the formation and build-up of deposits.

The invention relates to a method for obtaining cellulose fibres from fibrous biomass, in which: the biomass is first subjected to thermo-pressure hydrolysis, preferably with steam explosion, in a thermo-pressure hydrolysis plant, and then separation of the fibrous sludge obtained from the thermo-pressure hydrolysis plant is carried out in at least one separation plant, wherein a press cake of cellulose fibres, preferably with a dry material content of over 20%, preferably of over 25%, and a filtrate of flowable, solids-rich thin sludge are obtained, and wherein the thin sludge is fed to a biogas plant as a fermentation substrate to obtain biogas. The invention also relates to a plant for carrying out this method.

The present disclosure generally relates to a method for treatment of a lignocellulosic biomass. The method comprises the steps of pretreating the lignocellulosic biomass material in a pretreatment arrangement to form a pretreated biomass slurry; optionally hydrolysing the pretreated biomass slurry in a hydrolysis unit; retrieving gaseous SO2 from the pretreatment arrangement to provide an SO2 gas stream; treating the SO2 gas stream with an alkaline solution to provide a sulfite/bisulfite solution; supplying at least a part of the sulfite/bisulfite solution to at least one of: a) the lignocellulosic biomass material prior to pretreating the lignocellulosic biomass material in the pretreatment arrangement; b) the lignocellulosic biomass material n the pretreatment arrangement; c) the pretreated biomass slurry discharged from the reactor vessel; d) the hydrolysate or fermentable sugars of the hydrolysate.

The present invention provides automated devices for use in supporting various cell therapies and tissue engineering methods. The present invention provides an automated cell separation apparatus capable of separating cells from a tissue sample for use in cell therapies and/or tissue engineering. The cell separation apparatus can be used in combination with complementary devices such as cell collection device and/or a sodding apparatus to support various therapies. The automated apparatus includes media and tissue dissociating chemical reservoirs, filters, a cell separator and a perfusion flow loop through a graft chamber which supports a graft substrate or other endovascular device. The present invention further provides methods for using the tissue grafts and cell samples prepared by the devices described herein in a multitude of therapies including revascularization, regeneration and reconstruction of tissues and organs, as well as treatment and prevention of diseases.

The invention relates to a method for treating biomass (2). Biomass (2) is fed to a pressurized prehydrolysis reactor unit (8) by means of a feeding system (5, 7), wherein by means of the feeding system (5, 7) the biomass (2) is compressed. A filtrate is squeezed out of the biomass (2) by means of the feeding system (5, 7), in particular by a first plug screw (5) or a second plug screw (7) of the feeding system (5, 7). The biomass (2) is then thermally treated in the pressurized prehydrolysis reactor unit (8), discharged from the pressurized prehydrolysis reactor unit (8) afterwards, diluted with the filtrate before or after the discharge, and treated with an enzyme subsequently.

Method for producing bioproducts from streams of organic material, comprising the following steps: (i) the physical-biological pre-treating of the organic stream with water and one or more mechanical steps; (ii) setting the pH value of the mixture between pH 5 and pH 9; iii) adding an inoculum of a natural anaerobic culture that releases organic compounds; iv) a first separation step which splits the mixture into a solid and a liquid fraction, after which an anaerobic fermentation processes the solid fraction, with formation of biogas and digestate; v) an aerobic treatment of the separated liquid fraction with biological conversion of the organic compounds to a protein-rich bioproduct; vi) a second separation step with separation of the formed protein-rich bioproduct; vii) recirculating the liquid phase; viii) drying the formed protein-rich bioproduct, such that in the end a dry, protein-rich bioproduct is obtained as well as the bioproducts biogas and digestate.

A biological material manufacturing device according to an embodiment of the inventive concept includes a main body and a head unit that is rotatable on the main body. The main body includes a main groove and a first container groove connected to the main groove. The head unit includes a pillar provided in the main groove and a protruding part that protrudes from the pillar.

The present invention relates to a method for mechanically separating tissue and cells, and more specifically, to a method for mechanically separating tissue and cells, the method comprising the steps of: preparing adipose tissue; diluting the adipose tissue with a diluent to prepare an adipose tissue mixed solution; micronizing the adipose tissue, and various tissue and cells constituting the adipose tissue, in the adipose tissue mixed solution; and separating the adipose tissue and the various tissue and cells constituting the adipose tissue, in the adipose tissue mixed solution.

A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues.

The present disclosure relates to processes for producing hydrocarbon fuels from lignocellulosic biomass. A process may include introducing biomass to a pretreatment system, and a first separation system forming a pentose-rich stream and a pentose-lean stream. The pentose-lean stream may be introduced to a hydrolysis system forming a hydrolysate and the hydrolysate introduced to a second separation system forming a hexose-rich stream and a lignin stream. Additionally, at least one of the pentose-rich stream or the hexose-rich stream may be introduced to a bioreactor containing microorganisms configured to produce hydrocarbon fuels. Additionally, the present disclosure also relates to systems for the production of hydrocarbon fuels. A system may include a pretreatment system, a first separation system, a hydrolysis system, a second separation system, and one or more bioreactors. Alternatively a system may include a pretreatment system, a hydrolysis system, a sugar separation system, and one or more bioreactors.