The present disclosure provides methods of evaluating a therapeutic agent for cancer, and methods of cancer treatment.

The present invention relates to a method, which patterns 3D organoids prepared from human pluripotent stem cells and chops the same so as to culture oligodendrocyte progenitor cells, and induces the differentiation thereof so as to obtain a large quantity of finally differentiated oligodendrocytes. Compared to cells differentiated by a conventional differentiation method, oligodendrocytes obtained in a large quantity have the same or superior reproducibility, stability, and functionality and have remarkably shortened differentiation time, and thus are expected to be very useful for cell therapeutic agents or for screening for therapeutic drugs.

A method for producing a cell construct including, contacting Schwann cells or Schwann cell-like cells with a biocompatible matrix, and subjecting to cultivation, where the cultivation is at least partially performed by administering mechanical stimulation on the cells in contact with the biocompatible matrix. A cell construct obtained by the method.

The present disclosure provides compositions and methods for culturing hematopoietic stem and progenitor cells, while maintaining or increasing the subpopulation of hematopoietic stem cells (HSCs). The methods and compositions describe us a 3D zwitterionic hydrogel to provide a biocompatible culture microenvironment for culturing encapsulated hematopoietic stem and progenitor cells.

The present invention relates to a method for the large-scale synthesis of an edible and hot steam sterilizable macroporous three-dimensional (3D) scaffold which comprises biocompatible polymers with interconnected pores as a support material for adherent cell growth, proliferation and differentiation, which may be used to obtain tissue with nutritive content and/or cultured meat. These scaffolds are suitable for supporting cell tissue growth for biomedical or food applications.

A cell culture substrate having a high cell-adhesion portion and a low cell-adhesion portion, wherein; an adhesiveness to a cell of the high cell-adhesion portion and an adhesiveness to a cell of the low cell-adhesion portion are different from each other, the adhesiveness to the cell of the high cell-adhesion portion to cells is higher than the adhesiveness of the low cell-adhesion portion to the cell; and the high cell-adhesion portion has a cell adhesion layer containing two or more kinds of cell adhesion substances on the surface.

The present invention is directed to a system including neurons over-expressing UBB+1, organized in a 3-dimensional culture, and method of using same. A process for making the system of the invention is also provided.

The present invention provides a cell culture substrate including a polymer having a lower critical solution temperature, the substrate including one or more inorganic materials selected from a water-swellable clay mineral and silica and further including an adhesive matrix in the substrate, in which the adhesive matrix is an extracellular matrix and/or an adhesive synthetic matrix. Furthermore, the invention is to provide a cell culture substrate in which the extracellular matrix is at least one selected from laminin, fibronectin, vitronectin, cadherin, and fragments thereof, and/or the adhesive synthetic matrix is poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl) ammonium hydroxide] or an oligopeptide-supporting polymer.

Disclosed is a method of producing reconstructed human skin, and particularly a method of producing reconstructed human skin using a culture vessel including an inner chamber surrounded by an inner wall and a porous bottom surface and an outer chamber spaced apart from the inner chamber and configured to surround the inner chamber, including forming an adhesive layer by coating the inner wall with an adhesive material and culturing reconstructed human skin in the culture vessel having the adhesive layer formed thereon.

The present disclosure provides a substrate for cell culture. Systems comprising the substrate, and methods for using and manufacturing the substrate are also disclosed herein.