Microbial cell lines suitable for industrial-scale production of organic acids and methods of making and isolating such cell lines.

The present invention relates to a genetic tool comprising at least two different nucleic acids allowing the transformation, by homologous recombination, of a bacterium of the genus Clostridium, typically of a solventogenic bacterium.

Control processes are provided for enhancing biological processes for converting gaseous substrates to useful end products such as ethanol. A process includes providing a gaseous substrate comprising one or more of CO and CO.sub.2 to a bioreactor at a substrate addition rate. Acetogenic bacteria provided to the bioreactor ferment the gaseous substrate in a fermentation broth. The process includes determining a concentration of carboxylic acids and/or carboxylates in the fermentation broth. A control algorithm uses those concentrations to adjust the gaseous substrate addition rate.

The present disclosure relates to a composition for preparing hexanol or butanol and a method for preparing hexanol or butanol using ethanol and synthesis gas, wherein the composition according to an aspect of the present disclosure is a medium composition containing ethanol as an active ingredient, and by culturing a strain producing hexanol or butanol after inoculating with a medium containing the composition and supplying synthesis gas, hexanol or butanol can be prepared economically using inexpensive synthesis gas, and hexanol or butanol can be prepared with high efficiency by focusing the flow of a carbon source consumed in a fermentation process to the production of hexanol or butanol.

The present invention concerns a genetic tool comprising at least two distinct nucleic acids optimized to facilitate the transformation and modification by homologous recombination of a bacterium of the genus Clostridium, typically a solventogenic bacterium.

The present invention provides products and methods for improving the tolerance of a dough in bakery and patisserie products. More specifically it provides sourdough products obtained through the fermentation of cereals by specific strains of bacteria. The products obtained by using said sourdough products are characterized by having a strengthened dough resulting in bakery and patisserie products with improved physical properties and taste.

Provided is a device for continuously manufacturing an organic substance with high productivity, in which a means is installed whereby a synthesis gas having a specific nutrient source concentration for microbes in a continuous fermenter is stored with good efficiency and the gas composition thereof is made uniform in a stage prior to supplying the synthesis gas to the fermenter including microbes, so that uniformity of the synthesis gas composition is ensured. A device for manufacturing an organic substance, provided with a gas generating unit for gasifying a carbon source and generating a starting material gas, a gas supply unit for supplying a synthesis gas including the starting material gas to a fermenter, and a fermenter for microbial fermentation of the synthesis gas in the fermenter, a gas circulation unit for making the gas composition uniform being further provided between the gas generating unit and the gas supply unit or to the gas supply unit.

Microbial cell lines suitable for industrial-scale production of organic acids and methods of making and isolating such cell lines.

The present disclosure provides a novel and practical alcohol and derivatives thereof which have more industrial value than existing petrochemical raw materials. The present disclosure further provides ethanol, characterized in that a peak in gas chromatography measured by gas chromatograph spectrometry (GC/MS) has at least one peak with a retention time selected from (A) a peak of 5 minutes 25 seconds to 5 minutes 35 seconds and two peaks of 2 minutes 55 seconds to 3 minutes 5 seconds; (B) a peak of 12 minutes 30 seconds to 12 minutes 40 seconds; (C) a peak of 6 minutes 36 seconds to 6 minutes 45 seconds; and (D) a peak of 15 minutes 00 seconds to 15 minutes 15 seconds.

Control processes are provided for enhancing biological processes for converting gaseous substrates to useful end products such as ethanol. A process includes providing a gaseous substrate comprising one or more of CO and CO.sub.2 to a bioreactor at a substrate addition rate. Acetogenic bacteria provided to the bioreactor ferment the gaseous substrate in a fermentation broth. The process includes determining a concentration of carboxylic acids and/or carboxylates in the fermentation broth. A control algorithm uses those concentrations to adjust the gaseous substrate addition rate.