The present invention deals with the formation of Natural Organic Nano-Fertilizers with the chelated nano-nutrients to balance plant nutrition; improve water holding capacity, soil health improvement, sustainable productivity and quality improvement. The present invention involves production of eco-friendly and low cost process for the synthesis of nanoparticles of nano-nutrients by utilizing microorganisms comprising of two lab adapted strains of Aspergillus species, one lab adapted strain of Lactobacillus sp. and lactate, gluconate and proteinate salts as a source of nutrient leads to formation of metal nanoparticles (Zn, Mg, Fe and P) with the size of <20 nms. The present invention increases 12-20% of crop yield, stress tolerance of the crops, nutrient mobilization increases and 3 fold increase in nutrient use efficiency.

An active polysaccharide compound nutrient for boosting immunity and relieving fatigue, including 10-12 parts by weight of lentinan, 8-10 parts by weight of laminarin, 5-8 parts by weight of Lycium barbarum, 10-15 parts by weight of Mytilus edulis extract, 10-15 parts by weight of organic selenium protein powder, 8-10 parts by weight of walnut kernel, 3-5 parts by weight of black soybean powder, 2-3 parts by weight of Panax quinquefolius extract, 1-2 parts by weight of Schisandra chinensis, 1-2 parts by weight of Ophiopogon japonicus, 1-2 parts by weight of nutritional yeast, 5-10 parts by weight of L-arabinose, 5-8 parts by weight of xylitol and 0.2-0.3 part by weight of pancreatin. The active polysaccharide compound nutrient is prepared by subjecting raw materials to crushing, mixing, stirring, dissolving with deionized water, concentrating and drying under reduced pressure.

A traditional Chinese medicine polysaccharide extract freeze-drying protective agent, a direct-to-vat starter and preparation methods thereof are disclosed, which belong to the technical field of feed starter. The compound traditional Chinese medicine polysaccharide extract freeze-drying protective agent includes Poria cocos polysaccharide extract, Atractylodes macrocephala polysaccharide extract, Radix codonopsis polysaccharide extract, L-tyrosine, α-cyclodextrin and polyethyleneimine. Its application in the freeze-drying protection of direct-to-vat starter of aquatic fermentation feed can reduce the damage to the cells caused by freeze-drying, improve the survival rate of the cells in the starter after freeze-drying, effectively prolong the storage period of the starter, and ensure that the fermentation activity remains at a high level during storage.

The present invention relates to the field of genetic engineering, particularly to a glucose oxidase mutant having improved thermal stability, gene and application thereof. The present invention provides several glucose oxidase GOD mutants with high catalytic efficiency and improved thermal stability, which breaks the barrier of low enzyme activity and poor stability and is suited well to meet the requirements of application to the fields of food, medicine, feed and textile industry, and has a very broad application prospect.

The disclosure discloses an Aspergillus niger engineered strain for reducing byproduct fumaric acid in a fermentation process of L-malic acid. The Aspergillus niger engineered strain is an Aspergillus niger engineered strain in which a fumarate hydratase gene fum is knocked out. The disclosure overcomes the defects in the prior art, in the current process of producing malic acid through fermentation of Aspergillus niger, byproduct fumaric acid can be accumulated with the generation of malic acid so as to cause the improved cost of the subsequent malic acid purification process. The disclosure provides an Aspergillus niger engineered strain in which a fum gene is knocked out and a method for greatly reducing byproduct fumaric acid in the fermentation production of Aspergillus niger.

The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) an enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5′-phosphate as a coenzyme.

The present invention provides a method of preparing a furan derivative comprising the steps of (a) converting a monosaccharide to provide a keto-intermediate product; and (b) dehydrating the keto-intermediate product to provide a furan derivative; wherein the keto-intermediate product is pre-disposed to forming keto-furanose tautomers in solution. The method may further comprising a step of oxidizing the furan derivative to provide a furandicarboxylic acid or a furandicarboxylic acid derivative.

The present disclosure discloses a visualized screening method for an Aspergillus recombinant strain with multigene editing and belongs to the technical field of gene engineering. CRISPR-Cas9 is used in the disclosure to cleave spore color change-related genes and a target gene in Aspergillus at the same time, such that editing of the target gene is visualized and an Aspergillus niger strain with multigene editing can be rapidly and efficiently screened out through spore phenotypes. Through different combinations of visualized genes and non-phenotypic change genes, rapid screening of the strain with multigene editing and simultaneous screening of multiple visualized genes are realized, and use of resistance genes in industrial strains is reduced.

The Notice states that an abstract of the technical disclosure is required. In response, Applicant submits herewith a Preliminary Amendment including an abstract in compliance with 37 CFR § 1.72(b). The abstract is based on that submitted in parent U.S. application Ser. No. 15/556,084 (issued as U.S. Pat. No. 11,193,106) and international application no. PCT/US2016/020621, of which U.S. application Ser. No. 15/556,084 is the US national stage application. The submitted abstract differs from the abstract of the parent application only by a correction of “may further comprising” to “may further comprise”. Thus, the abstract contains no new matter.

A technique to desalinate seawater using melanin-concentrated solar energy wherein the melanin is extracted from a local isolate Aspergillus niger. A device consists of two fixed upper and lower containers with same volume of seawater in both, with or without melanin powder dissolved in the lower container at rate of 0.17 gm of melanin powder per 10 ml of water. The device is put outdoors under direct sunlight during daytime, circular water droplets free of salt starts to appear on the external bottom of upper container. Water droplets are collected by a sterile glass rod, pH of droplets water is about 7.1. Yield of fresh water is approximately 10 ml droplets water from 600 ml seawater per hour; after 24 hours day and night incubation, seawater in the upper container dries out leaving salt crystals. Yield of 1000 m3 seawater is 100 m3 freshwater (1000 L seawater yield 100 L freshwater).