The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

The present invention relates to a compound of general formula I ##STR00001## The present invention also relates to a method of producing N-acetyl homoserine and/or derivatives thereof, the method comprising contacting at least one recombinant cell in an aqueous medium with acetate wherein the recombinant cell comprises an increased activity relative to a wild type cell of (a) an enzyme E.sub.1, a homoserine dehydrogenase (EC1.1.1.3) and/or an enzyme E.sub.5, an aspartokinase (EC2.7.2.4); and (b) an enzyme E.sub.2, a homoserine O-acetyl transferase (EC2.3.1.31)
and the acetate is maintained at a concentration of at least about 0.001 g/L in the aqueous medium.

The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

A method is described for producing L-methionine by fermentation using a bacterium which has been modified to overexpress a 1st gene and a 2nd gene, such as genes having the nucleotide sequences shown in SEQ ID NOs: 1 and 3.

The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

The present invention relates to a compound of general formula I

##STR00001##

The present invention also relates to a method of producing N-acetyl homoserine and/or derivatives thereof, the method comprising contacting at least one recombinant cell in an aqueous medium with acetate wherein the recombinant cell comprises an increased activity relative to a wild type cell of (a) an enzyme E.sub.1, a homoserine dehydrogenase (EC1.1.1.3) and/or an enzyme E.sub.5, an aspartokinase (EC2.7.2.4); and (b) an enzyme E.sub.2, a homoserine O-acetyl transferase (EC2.3.1.31)
and the acetate is maintained at a concentration of at least about 0.001 g/L in the aqueous medium.

The present invention relates to a polypeptide having a resistant to feedback inhibition by methionine and an activity of homoserine O-succinyltransferase, a O-succinyl homoserine-producing microorganism expressing the polypeptide, and a method for producing O-succinyl homoserine using the same.

The present invention relates to a polypeptide which is resistant to feedback inhibition by methionine and has an activity of homoserine O-succinyltransferase, a microorganism for producing O-succinylhomoserine which expresses the polypeptide, and a method for producing O-succinylhomoserine using the same.

The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.