A genome-editing complex for modifying a target polynucleotide comprising a recombinant β helical protein linked to either one or more molecules of a genome-editing system or a plasmid encoding for one or more molecules of a genome-editing system, wherein the β helical protein length is in the range of from 5 nm to 25 nm, and width is in the range of from 1 nm to 5 nm.

Provided herein include methods and compositions for synthesis of Lipid IV.sub.A and derivatives thereof, using a defined set of pathway enzyme, which may be isolated and used to reconstitute all or part of the pathway in a cell-free reaction.

Provided herein include methods and compositions for synthesis of Lipid IV.sub.A and derivatives thereof, using a defined set of pathway enzyme, which may be isolated and used to reconstitute all or part of the pathway in a cell-free reaction.

A genome-editing complex for modifying a target polynucleotide comprising a recombinant helical protein linked to either one or more molecules of a genome-editing system or a plasmid encoding for one or more molecules of a genome-editing system, wherein the helical protein length is in the range of from 5 nm to 25 nm, and width is in the range of from 1 nm to 5 nm.

A genome-editing complex for modifying a target polynucleotide comprising a recombinant helical protein linked to either one or more molecules of a genome-editing system or a plasmid encoding for one or more molecules of a genome-editing system, wherein the helical protein length is in the range of from 5 nm to 25 nm, and width is in the range of from 1 nm to 5 nm.