The present invention is concerned with materials and methods for the production of genetically modified plants, particularly where the plants are for the production of at least one unsaturated or polyunsaturated fatty acid. The invention is also concerned with identification of genes conveying an unsaturated fatty acid metabolic property to a plant or plant cell, and generally relates to the field of phosphotidylcholine:diacylglycerol cholinephosphotransferase (PDCT).

The present invention relates to Brassica juncea ROD1 nucleic acid sequences and proteins and the use thereof to create plants with increased levels of C18:1 and reduced levels of saturated fatty acids in the seeds.

The present invention is concerned with materials and methods for the production of genetically modified plants, particularly where the plants are for the production of at least one unsaturated or polyunsaturated fatty acid. The invention is also concerned with identification of genes conveying an unsaturated fatty acid metabolic property to a plant or plant cell, and generally relates to the field of phosphotidylcholine:diacylglycerol cholinephosphotransferase (PDCT).

The present invention is concerned with materials and methods for the production of genetically modified plants, particularly where the plants are for the production of at least one unsaturated or polyunsaturated fatty acid. The invention is also concerned with identification of genes conveying an unsaturated fatty acid metabolic property to a plant or plant cell, and generally relates to the field of phosphotidylcholine:diacylglycerol cholinephosphotransferase (PDCT).

The present invention relates to soybean ROD1 nucleic acid sequences and proteins and the use thereof to create plants with increased levels of C18:1 and reduced levels of saturated fatty acids in the seeds.

Compositions and methods for producing plants with enhanced oil content and higher seed yield are disclosed. The transgenic plant comprises a polynucleotide encoding a monoacylglycerol O-acyltransferase 1 (MGAT1) operatively linked to a plant-expressible promoter; a polynucleotide encoding a phosphatidylcholine diacylglycerol cholinephosphotransferase 1 (PDCT1) operatively linked to a plant-expressible promoter; a polynucleotide encoding a suppressor of expression of Sugar Dependent 1 (SPD1) operatively linked to a plant-expressible promoter; a polynucleotide encoding a diacylglyerol acyltransferase (DGAT1) operatively linked to a plant-expressible promoter and a polynucleotide encoding a glycerol-3-phosphate dehydrogenase (GPD1) operatively linked to a plant-expressible promoter; or a combination thereof.

Aspects of the invention provide methods for differential regulation of fatty acid unsaturation in seed oil and membrane lipids of plants based on modulation of a previously unknown biosynthetic pathway involving a novel phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT) that regulates phosphatidylcholine biosynthesis in developing oil seed plants. Specific aspects relate to inventive PDCT polypeptides including, for example, variants, deletions, muteins, fusion proteins, and orthologs thereof (collectively PDCT proteins), to nucleic acids encoding same, to plants comprising such PDCT sequences or proteins or devoid or depleted of such PDCT proteins or sequences, and to methods for generating plants having altered or no PDCT expression and/or activity, including but not limited to methods comprising mutagenesis, recombinant DNA, transgenics, etc.

The present invention relates to Brassica plants comprising mutant phosphatidylcholine:diacylglycerol cholinephosphotransferase encoding (ROD1) genes, ROD1 nucleic acid sequences and proteins, as well as methods for generating and identifying said plants and alleles, which can be used to plants with increased levels of C18:1 in the seeds.

The present invention relates to soybean ROD1 nucleic acid sequences and proteins and the use thereof to create plants with increased levels of C18:1 and reduced levels of saturated fatty acids in the seeds.

Compositions and methods for producing plants with enhanced oil content and higher seed yield are disclosed. The transgenic plant comprises a polynucleotide encoding a monoacylglycerol O-acyltransferase 1 (MGAT1) operatively linked to a plant-expressible promoter; a polynucleotide encoding a phosphatidylcholine diacylglycerol cholinephosphotransferase 1 (PDCT1) operatively linked to a plant-expressible promoter; a polynucleotide encoding a suppressor of expression of Sugar Dependent 1 (SPD1) operatively linked to a plant-expressible promoter; a polynucleotide encoding a diacylglyerol acyltransferase (DGAT1) operatively linked to a plant-expressible promoter and a polynucleotide encoding a glycerol-3-phosphate dehydrogenase (GPD1) operatively linked to a plant-expressible promoter; or a combination thereof.