In some examples, an apparatus can include a light transmitter, a light sensor aligned along a light transmittance axis of the light transmitter, an impeller positioned between the light transmitter and the light sensor. The impeller can in some examples include a blade to pass through the light transmittance axis during rotation of the impeller. The blade can in some examples be translucent to permit calibration of the light sensor based on a comparison of a first light sensor reading when the blade intersects the light transmittance axis and a second light sensor reading when the blade does not intersect the light transmittance axis.

A method of optical analysis comprises receiving light at an optical spectrometer module from a light source, distributing the received light into two or more light beams with a light distribution component of the optical spectrometer module, concurrently exposing each of a reference and one or more test samples to one of the two or more light beams, and concurrently measuring a property of the light associated with each of the reference sample and one or more test samples with a corresponding detector.

Method for calibrating or monitoring performance of an optical measurement device that includes using a robotic arm to move a reference device having an optical reference material into a signal-detecting position of the optical measurement device. With the optical measurement device, detecting an emission emitted by the optical reference material of the reference device in the signal-detecting position. Then generating a reference signal representing a characteristic of the emission detected by the optical measurement device and comparing the reference signal to an expected reference signal for the emission to calibrate or monitor the performance of the optical measurement device.

This relates to systems and methods for measuring a concentration and type of substance in a sample at a sampling interface. The systems can include a light source, optics, one or more modulators, a reference, a detector, and a controller. The systems and methods disclosed can be capable of accounting for drift originating from the light source, one or more optics, and the detector by sharing one or more components between different measurement light paths. Additionally, the systems can be capable of differentiating between different types of drift and eliminating erroneous measurements due to stray light with the placement of one or more modulators between the light source and the sample or reference. Furthermore, the systems can be capable of detecting the substance along various locations and depths within the sample by mapping a detector pixel and a microoptics to the location and depth in the sample.

A system for monitoring reactions with a plurality of receptacle vessels that includes: an incubator; a movable receptacle carrier contained within a temperature-controlled chamber of the incubator; one or more fixed fluorometers configured to measure a fluorescent emission and positioned with respect to the receptacle carrier to measure fluorescent emissions from receptacle vessels carried on the receptacle carrier into an operative position with respect to each fluorometer; one or more fluorescent reference standards mounted on the receptacle carrier; and a controller configured to control operation of the receptacle carrier and the one or more fluorometers to determine if a fluorescent emission intensity of one or more of the fluorescent reference standards deviates from an expected fluorescent emission intensity.

An optical sensing system includes at least one electro-optical sensor having an adjustable field of view and at least one reflective member including a diffuse reflector surface positioned within the field of view of the at least one electro-optical sensor. The system also includes at least one controller configured to generate calibration parameters for the at least one electro-optical sensor based on data for at least one exposure detected by the electro-optical sensor when the diffuse reflector surface is within the field of view of the at least one electro-optical sensor. Methods for calculating the calibration parameters and for directly measuring reflectivity of objects in a scene with at least one electro-optical sensor are also disclosed herein.

A spectrum analysis apparatus is an apparatus for analyzing an analysis object on the basis of a spectrum of light generated in the analysis object containing any one or two or more of a plurality of reference objects, and includes an array conversion unit, a processing unit, a learning unit, and an analysis unit. The array conversion unit generates two-dimensional array data on the basis of a spectrum of light generated in the reference object or the analysis object. The processing unit includes a deep neural network. The analysis unit causes the array conversion unit to generate the two-dimensional array data on the basis of the spectrum of light generated in the analysis object, inputs the two-dimensional array data to the deep neural network, and analyzes the analysis object on the basis of data output from the deep neural network.

An intensity distribution acquirer acquires a first detected intensity distribution detected by a detector with a sample position at which a sample is not present irradiated with light by a standard light source, and acquires a second detected intensity distribution detected by the detector with the sample position at which a sample is not present irradiated with light by an irradiation light source during a first measuring work. An irradiation intensity calculator calculates a radiation intensity at each wavelength of irradiation light of the irradiation light source based on the first detected intensity distribution, the second detected intensity distribution and radiation characteristics of the standard light source. An irradiation photon count calculator calculates an irradiation photon count at each wavelength of irradiation light of the irradiation light source based on an irradiation intensity at each wavelength.

A concentration measurement method performed in a concentration measurement device including an electric unit having a light source and a photodetector, a fluid unit having a measurement cell through which a gas flows, and a processing circuit for calculating a concentration of the gas based on an intensity of a light passing through the measurement cell. The concentration measurement method includes a step of determining an absorption coefficient of the measurement gas using a reference absorption coefficient determined in association with the reference gas and a correction factor associated with the measurement gas, and a step of obtaining a concentration of the measurement gas flowing in the measurement cell using the absorption coefficient of the measurement gas. When the absorption peak wavelength of the measurement gas is longer than the peak wavelength of the light source, a reference gas having a longer absorption peak wavelength than the peak wavelength of the light source is used, and when the absorption peak wavelength of the measurement gas is shorter than the peak wavelength of the light source, a reference gas having a shorter absorption peak wavelength than the peak wavelength of the light source is used.

A fluid analyzer (214) that analyzes a sample (12) includes an analyzer frame (236); a test cell assembly (242) that receives the sample (12); a laser assembly (238) that generates a laser beam (239A) a signal detector assembly (232) and a self-check assembly (230). The self-check assembly (230) includes (i) a check frame (230A); (ii) a check substance (230E) with known spectral characteristics; and (iii) a check frame mover (230B) that selectively moves the check frame (230A) between a self-check position (231 B) and a test position (231 A) relative to the analyzer frame (236). In the self-check position (231 B), the laser beam (239A) is directed through the check substance (230E) to evaluate the performance of the fluid analyzer (214). In the test position (231 A), the laser beam (239A) is directed through the sample (12) in the test cell assembly (242) to evaluate the sample (12).