A solution is proposed for testing a luminescence imaging apparatus (105). A corresponding method (700) comprises acquiring (706) a photograph image and finding (708) a position of the testing device (110) in the photograph image. The 5method further comprises acquiring (706) a luminescence image and determining (734-736) a representation of sites of the testing device (110), each comprising at least one luminescence substance, in the luminescence image according to the position of the testing device (110) in the photograph image. The luminescence imaging apparatus (105) is then tested (754-772) according to the representation of the sites (330) in the 0luminescence image. A corresponding computer program (600) and a computer program product for implementing the method (700) are also proposed. Moreover, a testing device (110) for use in the method (700) is proposed.

A calibration target for calibrating an optoelectronic device for analyzing biomolecules by detecting fluorescence signals from a sample includes a substrate and a solid fluorescent layer that is disposed on the substrate and capable of being excited by laser light. The fluorescent layer has an optically inactive matrix having embedded therein a carbon-based component that is excitable to light emission.

The invention discloses a Trace Microanalysis Microscope System for high throughput screening. A multimodal imaging sensor arrangement acquires color, multispectral, hyperspectral and multi-directional polarized imaging, independently and in combinations thereof. In one aspect of this disclosure, the multimodal acquisition is combined with a plurality of sample illumination modes, further expanding the dimensionality of the generated data. In another aspect of this invention, machine learning-based methods combining and comparing a- priori data with the acquired multimodal data space, provide unique identifiers for the composition of the analyzed target objects. In yet another aspect of this invention, projection mapping of the identified compositional features navigates secondary sampling for subsequent analyses.

A method of calibrating an imaging device adapted to characterize a feature of a sample container, such as a cap color or cap type. The method includes providing a calibration tube including an imaging surface at an imaging location of a first imaging apparatus; illuminating the imaging surface with light emitted from multiple front light sources; adjusting a drive current to each of the multiple front light sources to establish a substantially uniform intensity of the imaging surface; recording drive current values for the multiple front light sources; replacing the calibration tube with a calibration tool having a calibration surface of a known reflectance; and measuring target intensity values of the calibration tool at the respective drive current values. Calibration tools, imaging apparatus, quality check modules, and health check methods are provided, as are other aspects.

A reference sample for calibrating and/or adjusting a microscope and to uses of the reference sample. The latter has at least one carrier structure made of a carrier material which, at least in regions of its extent, is excitable to emit luminescence light, and at least one two-dimensional and/or three-dimensional structure consisting of a number of substructures. The carrier material is diamond or silicon carbide and is doped in or around the regions of the two-dimensional and/or three-dimensional structure in order to be excitable to emit the luminescence light.

The disclosure provides a calibration assembly for a scan device. The calibration assembly includes a plurality of light-permeable plates and a reflection plate. The light-permeable plates are different in size, and the light-permeable plates are arranged along thicknesses directions thereof to form a step shape. The light-permeable plates define a plurality of light-permeable areas that respectively have different numbers of layers of the light-permeable plates inversely proportional to transmittances of the light-permeable areas. The light-permeable areas are configured to be permeable to a light having a predetermined frequency. The reflection plate is disposed at a side of one of the light-permeable plates in the thickness direction thereof. The reflection plate has a plurality of first holes having different sizes, and the reflection plate is configured to block the light having the predetermined frequency. The disclosure also provides a calibration system having the calibration assembly.

A method for true-to-sample measurement by a mobile ingredient analysis system having a housing with a window, an interface for an external reference unit, a display and operating unit, a light source, an optical spectrometer, a camera, an internal reference unit, and an electronic control unit. The method includes: selecting a calibration product suitable for a sample to be examined; performing a plausibility check of the calibration product, an incorrect selection being signaled and an alternative calibration product being selected; outputting measurement conditions comprising the measurement point to be selected and measurement duration for the selected calibration product; capturing measured values of the sample by the spectrometer under the measurement conditions and with simultaneous monitoring of the measurement conditions; processing the captured measured values by means of an electronic control unit, each measured value captured while the measurement conditions were met being declared valid; outputting the measured values deemed valid.

A system and method for calibration of a fluorometer using a calibration cell comprising a sealed container housing one or more calibration solutions of known fluorescence. The inventive calibration device includes a sealed calibration cell housed in a storage chamber that may be permanently or temporarily affixed to the top of a fluorometer such that the calibration solution can be moved by manual or automated means directly from the storage chamber to the fluorometer cell for a calibration operation. All of the solution needed for any given calibration can be contained exclusively, in small quantity, inside the calibration cell.

A method of forming an imaging calibration device for a biological material imaging system is provided. A first set of one or more discrete regions is provided upon or within a retaining member. Each said region of the first set comprises a selected tissue stain material, wherein each of the selected tissue stain materials exhibits a predetermined optical response inside a colour-stable region of a chromaticity diagram defined by the ellipse x2/a2+y2/b2=1, where a=0.21, b=0.135, the rotation is −0.07 rad and the translation is (0.38,0.333). A second set of one or more discrete regions is also provided upon or within the retaining member. The second set is formed of one or more coloured filters having a predetermined optical response outside the colour-stable region.

Provided by the inventive concept or nanoplastic or microplastic particles, reference standard materials including nanoplastic or microplastic particles, methods of using, and methods of preparing the same. Uses of the nanoplastic and/or microplastic particles of the inventive concept include tracking of nanoplastic and/or microplastic particle dispersion/distribution in environmental and/or biological systems, as well as in organisms that are within the environment.