Systems and methods are provided for a UV-VIS spectrophotometer, such as a UV-VIS detector unit included in a high-performance liquid chromatography system. In one example, a system for the UV-VIS detector unit may include a first light source, a signal detector, a flow path positioned intermediate the first light source and the signal detector, a second light source, and a reference detector. The first light source, the signal detector, and the flow path may be aligned along a first axis, and the second light source and the reference detector may be aligned along a second axis, different than the first axis.

A flow cell device including a spherical optical element is disclosed. The spherical lens can be sealed to the body of the flow cell device in a manner that provides external optical access to a fluid in an analysis region of a flow path through the flow cell device. The seal can be provided by an elastomer, a polymer, or a deformable metal. The disposition of the spherical lens to the flow path enables in situ optical analysis of the fluid. An optical analysis device can be removably connected to the flow cell device to provide the optical analysis. In some embodiments the optical analysis device is a portable Raman spectrometer. The flow cell device can provide a supplementary interrogation interface, and/or an on board sensor device(s) to enable multivariate analysis and/or advanced triggering.

A flow cell device including a spherical optical element is disclosed. The spherical lens can be sealed to the body of the flow cell device in a manner that provides external optical access to a fluid in an analysis region of a flow path through the flow cell device. The seal can be provided by an elastomer, a polymer, or a deformable metal. The disposition of the spherical lens to the flow path enables in situ optical analysis of the fluid. An optical analysis device can be removably connected to the flow cell device to provide the optical analysis. In some embodiments the optical analysis device is a portable Raman spectrometer. The flow cell device can provide a supplementary interrogation interface, and/or an on board sensor device(s) to enable multivariate analysis and/or advanced triggering.

An apparatus and method for an alignment cell are described herein. One apparatus includes a delivery fiber and a delivery lens coupled to an optical bench, a mirror to receive light from the delivery fiber through the delivery lens, wherein the received light is directed by the mirror to an ion trap on the trap surface, and a collection fiber coupled to the optical bench to receive light fluoresced from an ion in the ion trap.

An optical detecting device for detecting a nanoscale object, comprising: a microfluidic device, a microlens array, a light source and a light detecting element, wherein the microfluidic device comprises a top wall and a bottom wall arranged oppositely and a microfluidic channel between the top wall and the bottom wall; the microlens array is arranged on a surface of the bottom wall, and the bottom wall is made of an optically transparent material, and the light source is arranged on the surface of the bottom wall away from the microlens array aligned to the microlens array; the beam of the light source causes the formation of a photonic nanojet area in the microfluidic channel; the light detecting element receives light from the photonic nanojet area to detect the nanoscale object arranged in the photonic nanojet area.

A flow cell includes a housing, a window member, and a pressing member. The housing includes a cell channel in which a sample flows through, has, on at least one end side of the cell channel, an opening communicating with the cell channel, and has a flat surface at an edge of the opening. The window member has a lens portion at a central portion and a peripheral edge portion whose one surface and the other surface are flat. In the window member, one surface of the peripheral edge portion is provided facing the flat surface of the housing so as to seal the opening.

An apparatus and method for an alignment cell are described herein. One apparatus includes a delivery fiber and a delivery lens coupled to an optical bench, a mirror to receive light from the delivery fiber through the delivery lens, wherein the received light is directed by the mirror to an ion trap on the trap surface, and a collection fiber coupled to the optical bench to receive light fluoresced from an ion in the ion trap.

A toner detection unit (optical sensor) includes a first light receiving element configured to receive specular reflection light of first irradiating light, and a second light receiving element configured to receive diffused reflection light of second irradiating light, and a separation component configured to separate irradiating light emitted from an LED into the first and second irradiating light. A housing forms a first opening through which the first irradiating light and the second irradiating light pass and the diffused reflection light to be received by the second light receiving element passes, and a second opening through which the specular reflection light to be received by the first light receiving element passes.

Disclosed is a micro-lens imaging multi-well test plate which comprises: a transparent plate of 3-5 mm in thickness with one or more trapezoidal wells locating in the middle of the plate, each of the wells is of an underside of 2-4 mm in diameter, 0.2-0.5 mm in thickness, a trapezoidal dip angle of 60-75, and has a micro-lens which upper half is hemispherical, lower half is a cylinder, with radius of 0.11.0 mm, height of 0.22.5 mm, molded on the bottom of the well. The micro-lens imaging multi-well test plate is made of homogeneous optical transparent materials. When the trapezoidal concave wells of the test plate are filled with fluid to immerse the micro-lens, under parallel light illumination, due to the refraction effect of light, the image of micro-lens is a round one with an outer edge that is a black ring. The outer radius R of the black ring is the radius of the micro-lens, the inner radius r of the black ring is a function of the refractive index n.sub.1 of the immersion liquid, the refractive index n.sub.2 of the micro-lens and the height h of the micro-lens, so the refractive index of the sample fluid can be determined by monitoring the value of the inner radius r of the black ring with known values of R, n.sub.2 and h. By using a multi-well test plate for imaging, the individual refractive indices of different sample fluids in all the wells can be determined simultaneously in one measurement.

A water vapor distribution measurement apparatus comprises: a light source that emits near-infrared light; a near-infrared light measurement device that is located across a measurement space from the light source and that measures the near-infrared light; an optical system that expands and applies the near-infrared light emitted from the light source in the measurement space in which a cross-section of the measurement space perpendicular to a direction connecting the light source to the near-infrared light measurement device has an area; and a distribution deriving means for deriving a water vapor distribution in the cross-section of the measurement space on the basis of a measurement result obtained by the near-infrared light measurement device. Water vapor in a measurement region having a prescribed size can be measured by this water vapor distribution measurement apparatus.