The invention relates to a device (1) and a method for determining the action of active ingredients on nematodes and other organisms in aqueous tests. The device (1) according to the invention comprises a holder (13) for a cell culture plate (30) having multiple wells (31) in which the nematodes can be filled with the active ingredients, said cell culture plate (30) having a bottom side (33), a top side (32) and also side walls extending between bottom side (33) and top side (32), a camera (11) which is used to record images of preferably the bottom side (33) of the cell culture plate (30), a lighting mechanism (14) having at least a first light source (15) which illuminates the cell culture plate (30), there being arranged between the first light source (15) and a first side wall (34) of the cell culture plate (30) in the installed state a first optical unit which directs the light of the first light source (15) through the first side wall (34) in the direction of the bottom side (33) of the cell culture plate (30). The method according to the invention makes it possible to simultaneously investigate many active ingredients within a very short time.

This invention provides devices for use in various analytical applications including single-molecule analytical reactions. Methods for detecting analytes optically by propagating optical energy by waveguides within a substrate are provided. Analytical devices are provided which have both shallow and deep waveguides in which illumination light is transported through the deep waveguides and coupled into the shallow waveguides. The shallow waveguides provide evanescent field illumination to analytes, such as single-molecule analytes, within nanometer scale wells. Integrated devices including integrated detectors such as CMOS detectors are included.

A light distributing device configured for, in use, distributing, over a scene to illuminate light rays that come from an auxiliary light source, and which comprises: a planar waveguide, with a core layer disposed between the two cladding layers; and an extraction set, located in the planar waveguide, and constituted by a plurality of diffraction gratings distributed in the two dimensions of a plane parallel to the plane of the planar waveguide.

A photoplethysmographic (PPG) device is disclosed. The PPG device can include one or more light emitters and one or more light sensors to generate the multiple light paths for measuring a PPG signal and perfusion indices of a user. The multiple light paths between each pair of light emitters and light detectors can include different separation distances to generate both an accurate PPG signal and a perfusion index value to accommodate a variety of users and usage conditions. In some examples, the multiple light paths can include the same separation distances for noise cancellation due to artifacts resulting from, for example, tilt and/or pull of the device, a user's hair, a user's skin pigmentation, and/or motion. The PPG device can further include one or more lenses and/or reflectors to increase the signal strength and/or and to obscure the optical components and associated wiring from being visible to a user's eye.

Fourier Transformation Spectrometer, FT Spectrometer, comprising: A double beam interferometer, comprising: At least one beam splitter unit (622; 623; 624, 625, 626, 627; 636; 673, 674, 675) for splitting an incident light beam (EB) of a spatially expanded object into a first partial beam (TB1) and a second partial beam (TB2); at least a first beam deflection unit (630; 641; 651; 661; 697) designed to deflect the first partial beam (TB1) at least a first and a second time, wherein the second beam deflection unit (630) is designed to also deflect the second partial beam (TB2) at least at first and a second time; or the double beam interferometer comprises a second beam deflection unit (642; 652; 662) designed to deflect the second partial beam (TB2) at least a first and a second time, wherein the beam deflection unit is also designed to at least partially spatially overlay the first partial beam (TB1) and the second partial beam (TB2), and the respectively first and second deflection of the first partial beam (TB1) and of the second partial beam (TB2) generates a lateral shear (s); at least a first field of view discriminator unit (BFD1; 631; 645; 653; 656; 666; 677; 976) arranged such that the first partial beam (TB1) is spatially selected after the splitting and prior to the second deflection; at least a second field of view discriminator unit (BFD2; 632; 646; 654; 657; 667; 678; 977) arranged such that the second partial beam (TB2) is spatially selected after the splitting and prior to the second deflection.

An LED matrix lighting device for illuminating a lighting pattern with even intensity. The LED matrix lighting device includes a plurality of LEDs, a collimating lens in front of each LED for collimating light of the LED, and a light refracting element in front of collimating lenses arranged to refract light of at least a first part of the LEDs with a different refraction angle than at least a second part of the LEDs. The disclosure further relates to a machine vision system, a method, and a computer program product.

An optical gas concentration measurement apparatus is disclosed. The optical gas concentration measurement apparatus includes a thermally insulated enclosure that has a gas sample cell situated within. A thermally-insulating, light-guiding element passes through an access port of the thermally insulated enclosure and is configured to direct light from a light source outside of the thermally insulated enclosure to the gas sample cell. A light detector outside of the thermally insulated enclosure is optically coupled to the gas sample cell and an electronic assembly outside of the thermally insulated enclosure is configured to receive information from the light detector.

A method of microscopy comprises collecting an emission light; symmetrically dispersing the collected emission light into a first order (“1.sup.st”) light and a negative first order (“−1.sup.st”) light using a grating; wherein the 1.sup.st light comprises spectral information and the −1.sup.st light comprises spectral information; capturing the 1.sup.st light and the −1.sup.st light using a camera, localizing the one or more light-emitting materials using localization information determined from both the first spectral image and the second spectral image; and determining spectral information from the one or more light-emitting materials using the first spectral image and/or the second spectral image; wherein the steps of localizing and obtaining are performed simultaneously. A spectrometer for a microscope comprises a dual-wedge prism (“DWP”) for receiving and spectrally dispersing a light beam, wherein the DWP comprises a first dispersive optical device and a second dispersive optical device adhered to each other.

A fluorescence detection system, including apparatus and methods, suitable for qPCR and other fluorescence-based analyses. The system may comprise various components, including a stage, an illumination module, a detection module, and an optical relay structure. The stage may be configured to support a sample holder. The illumination module may include one or more discrete light sources configured to produce excitation light. The detection module may be configured to detect fluorescence emission light produced, in response to the excitation light, by a fluorescent sample positioned in the sample holder. The optical relay structure may include a beamsplitter assembly configured to direct the excitation light from the illumination module along an illumination path to the sample holder and to direct the fluorescence emission light from the sample holder along a response path to the imaging module. The system may enhance the quality of excitation light hitting samples in the sample holder.

Provided are a diffusion plate that diffuses light emitted from a light source, and a prism having a first surface to receive the light transmitted through the diffusion plate, a second surface to reflect the light in contact with a measurement target liquid, and a third surface to extract the reflected light. The light source, the diffusion plate, a light receiving lens, and an imaging element are accommodated in a holder that presses the prism from the inner side to the outer side.