A device configured to provide a value indicative of fluorescent emission from a substrate 5 having a test region is provided. The device comprises an electromagnetic radiation source configured to emit excitation radiation towards the test region to excite fluorescent emission from a fluorescent material in the test region. The electromagnetic radiation source is configured such that a variation in intensity of the excitation radiation across the test region is less than 15%. The device further comprises a sensor configured to capture a primary 10 image of the fluorescent emission, and a controller configured to modify the primary image based on calibration data and to use the modified image to obtain the value indicative of the fluorescent emission.

An optical measurement device includes an irradiation optical system, a detection optical system, and a cancel circuit. In a fluorescence detection process, a sample is designated as an irradiation target, the sample is irradiated with irradiation light, measurement target light including fluorescence generated from the sample irradiated with the irradiation light and light scattered from the sample irradiated with the irradiation light is detected as detection light, a signal component corresponding to the scattered light is removed from a measurement signal corresponding to the measurement target light in consideration of a result of performing a calibration process during a preliminary process. In the preliminary process, the calibration process for removing a signal component corresponding to the scattered light from the measurement signal is performed on the basis of a calibration signal having a higher signal intensity than a signal corresponding to the scattered light in the measurement signal.

A concentration measurement method performed in a concentration measurement device including an electric unit having a light source and a photodetector, a fluid unit having a measurement cell through which a gas flows, and a processing circuit for calculating a concentration of the gas based on an intensity of a light passing through the measurement cell. The concentration measurement method includes a step of determining an absorption coefficient of the measurement gas using a reference absorption coefficient determined in association with the reference gas and a correction factor associated with the measurement gas, and a step of obtaining a concentration of the measurement gas flowing in the measurement cell using the absorption coefficient of the measurement gas. When the absorption peak wavelength of the measurement gas is longer than the peak wavelength of the light source, a reference gas having a longer absorption peak wavelength than the peak wavelength of the light source is used, and when the absorption peak wavelength of the measurement gas is shorter than the peak wavelength of the light source, a reference gas having a shorter absorption peak wavelength than the peak wavelength of the light source is used.

The disclosure relates to systems and methods for controlling the pH of a sample, comprising measuring an initial pH, adding an amount of titrant and measuring a second pH, and using non-dimensional modeling to normalize titrant and determine the amount of titrant needed to reach a final pH. The systems and methods can be used to control pH during viral inactivation or titration of protein samples.

Methods and compositions are disclosed for preparing a predetermined standardized calibration curve, which can be stored on a label, barcode or tag. A lyophilized product containing assay reagents for use in a bioassay and a known amount of analyte. A predetermined calibration curve associated with the lyophilized product. A predetermined calibration curve makes the preparation of a new calibration curve unnecessary. The methods and compositions are useful in bioassays such as a FRET assay, a real-time reverse transcription polymerase (RT-PCR) assay, a chemiluminescence assay, or any other bioassay that elicits a detectable response based on a change in, or appearance of, color, fluorescence, or reflectance.

A method and system for calibrating a PET scanner are described. The PET scanner may have a field of view (FOV) and multiple detector rings. A detector ring may have multiple detector units. A line of response (LOR) connecting a first detector unit and a second detector unit of the PET scanner may be determined. The LOR may correlate to coincidence events resulting from annihilation of positrons emitted by a radiation source. A first time of flight (TOF) of the LOR may be calculated based on the coincidence events. The position of the radiation source may be determined. A second TOF of the LOR may be calculated based on the position of the radiation source. A time offset may be calculated based on the first TOF and the second TOF. The first detector unit and the second detector unit may be calibrated based on the time offset.

A calibration standard for determining an intensity decay related to an evanescent field generated close to the interface between a sample to be tested and a substrate on which the sample is to be deposited, preparation and analysis methods and use thereof.

Disclosed are a method and a device for measuring a sand content in a miscible phase fluid, the method comprising: flowing of the miscible phase fluid out of an oil and gas well through a pipeline, the miscible phase fluid of the wellhead of the oil and gas well including at least two fluid media; carrying out a measurement with a light quantum of four levels on the miscible phase fluid by a phase separator installed on the pipeline, such that a linear mass of each fluid medium is obtained; calculating a sand content in mass fraction based on the linear mass of all the fluid media, when the fluid media in the miscible phase fluid includes a solid phase sand.

A library of known intrinsic spectra is provided to identify at least one known material in a sample of interest. The library includes individual intrinsic spectra channels defined by the assignment of intrinsic spectra of at least one known material, and combinations thereof, so that the assigned intrinsic spectra of each intrinsic spectra channel is correlated to at least one known material. The at least one known material is identified in the sample of interest when intrinsic spectra obtained from the sample of interest is matched to an assigned intrinsic spectra of an intrinsic spectra channel of the library of known intrinsic spectra.

The present teachings relate to a method and system for normalizing spectra across multiple instruments. In an embodiment of the present invention, the method comprises at least one reference instrument and a test instrument. Each instrument comprises at least one excitation filter and at least one emission filter arranged in pairs. Each instrument further comprises a pure dye plate comprising a plurality of wells. Each well contains a plurality of dyes where each dye comprises a fluorescent component. Fluorescent spectra are obtained from each instrument for each dye across multiple filter combinations to contribute to a pure dye matrix Mref for the reference instrument and pure dye matrix M for the test instrument. The pure dye spectra can then be multiplied by correction factors for each filter pair to result in corrected pure dye spectra, then normalized and the multicomponenting data can be extracted.