A microfluidic device comprises a lower layer that is electrically conductive and transparent with respect to an incident optical beam, an upper layer, comprising first portions that are electrically conductive and second portions that are electrically insulating, adjacent and alternated to the first ones; a compartment seamlessly extending between the lower layer and the upper layer; the compartment contains a filler medium configured to emit an optical emission beam and markers dispersed in the filler medium, which are electrically charged and are adapted to move inside the compartment in all directions according to the intensity of the electrical signal applied to the first portions, the filler medium is configured to interact with the markers to increase or decrease the intensity of the optical emission beam according to the local concentration of the markers.

A glycemic management related analyte detecting assay device (10) and method are provided for detecting and quantifying analyte concentrations in a fluid sample. The assay device includes an absorbent body containing an assay forming a detection zone for receiving a fluid test sample. The absorbent body is provided in a chamber of the device. The assay can detect one or more of a glycemic analyte selected from the group consisting of fasting plasma blood glucose, oral glucose, % glycated hemoglobin, and fasting insulin concentrations. In one embodiment, a container includes an absorbent body having a plurality of superimposed membranes (30, 32, 34, 36) where each membrane contains a reactant and a color indicator for detecting the presence of a selected analyte above a predetermined concentration in the fluid sample. The absorbent body and/or the assay include a color indicator that is able to provide a visual indication of the presence of one or more glycemic analytes present in the test sample.

A strip structure for measuring potassium ions includes: a strip having an inner space for receiving a solution therein and being formed in a plate shape; an inlet formed in the strip and capable of injecting a solution into the inner space of the strip; a potassium ion selective membrane arranged in the inner space and capable of permeating potassium ions of the solution; and a first working electrode extending in a strip shape, wherein one side of the first working electrode is arranged inside the potassium ion selective membrane and the other side of the first working electrode is on a surface of the strip.

A system for analyzing a wound fluid. The system includes a transparent layer, a membrane layer, and an indicator layer that contains a colorimetric or fluorescent indicator reagent for detecting pH, a nitrite, an enzyme, a reactive oxygen species, a reactive nitrogen species, a nucleic acid, or a combination thereof. The membrane layer is impermeable to blood clots and cellular debris and is permeable to wound fluid. Also provided are methods for analyzing a wound fluid and for detecting biological fluid on biomedical instruments and waste materials using the system.

The application discloses a test strip and a method for manufacturing the test strip. The test strip comprises a base layer; an intermediate layer overlaid on the base layer; a blood retaining layer comprising a slit and a blood retaining region fluidly commuted with the slit and overlaid on the intermediate layer; an upper layer overlaid on the blood retaining layer; a reagent disposed on a surface of the intermediate layer and exposed to the slit, wherein there are an expectedly predetermined depth and a measured depth from an interface between the slit and the upper layer to an upper surface of the intermediate layer; and a classification mark representing a compensation factor and disposed on an upper surface of the upper layer or a lower surface of the base layer; wherein the compensation factor is the product of a difference between the predetermined depth and the measured depth and a reciprocal of the predetermined depth.

A rapid immunoassay method and apparatus for detecting foot and mouth disease virus are disclosed. The method and test device permit pen-side testing of animals and provide test results within a relatively short time period. In a preferred embodiment, the method and apparatus provide a means for differentiating between FMDV-infected and FMDV-vaccinated animals.

A moisture-responsive film can include a complexing substrate and an iodine layer applied to the complexing substrate to form the moisture-responsive film. The moisture-responsive film can include a test area formed on a testing surface of the moisture-responsive film.

A microfluidic device comprises a lower layer that is electrically conductive and transparent with respect to an incident optical beam, an upper layer, comprising first portions that are electrically conductive and second portions that are electrically insulating, adjacent and alternated to the first ones; a compartment interposed between the lower layer and the upper layer seamlessly extending between the lower layer and the upper layer; the compartment contains a filler medium that is transparent with respect to the incident optical beam and markers dispersed in the filler medium; the markers are electrically charged and are adapted to move inside the compartment in all directions in variable amounts according to the intensity of the electrical signal applied and to emit an optical emission beam when lit by an incident optical beam.

A sample testing apparatus for characterizing at least one target molecule in a testing sample includes a substrate and at least one detection device over the substrate. Each detection device includes a plurality of electrodes, a plurality of data lines, and a probe. Each electrode is configured, upon reaction of the probe with one of the at least one target molecule, to sense an electrical signal, and then to transmit the electrical signal via the one data line. Each data line includes a first film layer and at least one other film layer disposed over the first film layer. The first film layer can be at a substantially same layer, and have a first composition substantially same, as the electrodes. One or more of the at least one other film layer can have a composition having a relatively lower electric resistance than the first composition.

The present disclosure is drawn to loop-mediated isothermal amplification (LAMP) reaction assemblies including a substantially hygroscopic agent free LAMP reagent mixture in combination with a solid-phase reaction medium. The present disclosure also includes systems for a chromatic LAMP analysis including a substantially non-reactive solid phase reaction medium, and a non-interfering reagent mixture. The present disclosure also includes solid phase LAMP reaction mediums comprising a substrate, an adhesive layer disposed on the substrate, a reaction layer disposed on the adhesive layer, and a spreading layer disposed on the reaction layer. The present disclosure also includes methods of testing for a presence of a target nucleotide sequence including providing a biological sample, and dispensing the sample into a test environment having a solid phase reaction medium in combination with a LAMP reagent mixture and a pH sensitive dye.