A microscope system is provided with a microscope that acquires images at least at a first magnification and a second magnification higher than the first magnification, and a processor. The processor is configured to specify a type of a container in which a specimen is placed, and when starting observation of the specimen placed in the container at the second magnification, the processor is configured to specify an observation start position by performing object detection according to the type of container on a first image that includes the container acquired by the microscope at the first magnification, and control a relative position of the microscope with respect to the specimen such that the observation start position is contained in a field of view at the second magnification of the microscope.

Microscopic analysis of a sample includes a system using dark-field illumination. A mid-IR optical source generates a mid-infrared beam, which is directed onto the sample to induce a temperature change by absorption of the mid-infrared beam. A visible light source generates a light illuminating the sample on a substrate and creating a scattered field and a reflected field along a collection path of the system. A pupil mask is positioned along the collection path to block the reflected field while allowing the scattered field to pass therethrough. A camera is positioned at an end of the collection path to collect the scattered field and generate a dark-field image of the sample.

An immersion objective includes a correction group or correcting a spherical aberration. The displacement of the correction group along the optical axis leads to a substantially negligible defocus aberration.

The present disclosure provides a continuous zoom stereoscopic microscope with an adjustable stereoscopic angle, consisting of a microscope stand, a first eyepiece module, a second eyepiece module, a first objective module, a second objective module, a first Risley prism, a second Risley prism, a first image-rotating prism, a second image-rotating prism, a drive module, a control module and an illumination module. The drive module provides preset drive values for individual liquid lenses in the liquid lens sets according to different magnifications to change the focal lengths of the liquid lenses, thereby changing the effective focal lengths of the objective module and that of the eyepiece module, and finally achieving continuous and fast zooming of the stereoscopic microscope to be adapted to different working scenarios. The control module controls the relative angle between the two wedge-angle prisms in the Risley prism, achieving the continuous adjusting of the stereoscopic angle of the stereoscopic microscope, and then acquiring stereo images with different stereo senses.

Provided herein are systems and methods for simultaneous imaging and stimulation using a microscope system. The microscope system can have a relatively small size compared to an average microscope system. The microscope can comprise in part an imaging light source and a stimulation light source. Light from the imaging light source and the stimulation light source can be spectrally separated to reduce cross talk between the stimulation light and the imaging light.

A variable magnification optical system with boresight error correction includes a focusing lens to receive light along an optical axis of the variable magnification optical system, with the focusing lens configured to create an image of a target at a focal plane. The system includes a magnification changer disposed along the optical axis, with the magnification changer including an optomechanical drive system to adjust an optical magnification setting of one or more zoom lenses. The system also includes a light source configured to emit a pilot beam into the magnification changer. The system includes a position sensitive photodetector configured to receive the pilot beam exiting the magnification changer. The system further includes a microdisplay optically conjugate to the focal plane, with the microdisplay configured to impose an image of an electronic reticle on the focal plane based on the position of the pilot beam relative to the position sensitive photodetector.

A mobile phone-based dark field microscope (MDFM) apparatus suitable for quantifying nanoparticle signals is provided. The MDFM apparatus includes an electrically operated light source, a dark-field condenser, a slide housing configured to receive an analytical slide, and an adapter housing configured to receive an objective lens and receive a portable electronic communication device. The slide housing positions the analytical slide between the objective lens and the dark-field condenser. The adapter housing registers the objective lens with a camera lens of the portable electronic communication device. A method for performing a biological quantitative study using the dark-field microscope apparatus is further provided.

A method of automated measurement of motility and morphology parameters of the same single motile sperm. Automated motility and morphology measurements of the same single sperm are performed under different microscope magnifications. The same single motile sperm is automatically positioned and kept inside microscope field of view and in focus after magnification switch. A method of automated non-invasive measurement of sperm morphology parameters under high magnification of imaging. Sperm morphology parameters including subcellular structures are automatically measured without invasive sample staining. A method of automatically selecting sperms with normal motility and morphology and DNA integrity for infertility treatment.

The present disclosure relates to a medical microscope field. A stereo microscope connected to an optical coherence tomography (OCT) unit for forming a tomographic image of a target object includes an objective lens unit including a plurality of lenses each having an aperture of a predetermined size, a pair of first magnification lens units each including a plurality of lenses having a pair of magnification lens apertures positioned within the aperture of the objective lens unit, a second magnification lens unit including a plurality of lenses having an OCT aperture disposed separately from the pair of magnification lens aperture within the aperture of the objective lens unit, and a light delivery unit configured to receive light from the OCT unit and deliver the light to the second magnification lens unit and configured to deliver light received from the second magnification lens unit to the OCT unit.

Provided herein are systems and methods for imaging using a microscope system comprising removeable or replaceable component parts. Such systems and methods employ semi-kinetic coupling for easy, tool-free attachment of the microscope system to a baseplate. Systems and methods provided herein may comprise simultaneous imaging and stimulation using a microscope system. The microscope system can have a relatively small size compared to an average microscope system.