An apparatus and method to produce a hologram of an object includes an electromagnetic radiation assembly configured to receive a received electromagnetic radiation, such as light, from the object. The electromagnetic radiation assembly is further configured to diffract the received electromagnetic radiation and transmit a diffracted electromagnetic radiation. An image capture assembly is configured to capture an image of the diffracted electromagnetic radiation and produce the hologram of the object from the captured image.

The present disclosure provides improved systems and methods for automated cell identification/classification. More particularly, the present disclosure provides advantageous systems and methods for automated cell identification/classification using shearing interferometry with a digital holographic microscope. The present disclosure provides for a compact, low-cost, and field-portable 3D printed system for automatic cell identification/classification using a common path shearing interferometry with digital holographic microscopy. This system has demonstrated good results for sickle cell disease identification with human blood cells. The present disclosure provides that a robust, low cost cell identification/classification system based on shearing interferometry can be used for accurate cell identification. For example, by combining both the static features of the cell along with information on the cell motility, classification can be performed to determine the type of cell present in addition to the state of the cell (e.g., diseased vs. healthy).

The invention relates to a method of creation of three-dimensional alignment patterns that includes providing a layer of optically recordable and polarization sensitive material having a thickness that is greater than, or equal to, a predefined thickness, and concurrently illuminating the optically recordable medium with two coherent beam of same or different polarization with predetermined angle between the beams such that the said beams impinge from the same side or from the opposite sides upon the layer of the recordable material. The invention further relates to polarization volume holograms based on the said alignment patterns and polarization holographic element including a single layer or a stack of several layers of optically recordable materials containing single or multiple polarization volume holograms.

According to an embodiment, a holographic microscope comprises a light source emitting light to an object, a beam splitter reflecting the light emitted from the light source to the object and transmitting object light reflected from the object, a holographic image sensor sensing information, including a holographic image, by receiving the object light and allowing the object light to coherently interfere with reference light, and an image processor obtaining three-dimensional (3D) information about the object based on the information sensed by the holographic image sensor. The holographic image sensor includes a lens focusing the object light to the holographic image sensor, a filter transmitting a predetermined wavelength band of light of the focused object light, a light receiving unit receiving interference light to sense a holographic image, and a reference light source directly emitting the reference light having the predetermined wavelength band to the light receiving unit.

The present invention relates to a system and method for digital holographic microscopy. According to an aspect of the invention there is provided an off-axis digital holographic microscope comprising: a light emitter configured to provide a divergent light beam; a sensor position to receive light from the light emitter in a first path and a second path, and thereby to detect a holographic image; a reflector positioned partially in the divergent light beam so that light that encounters the reflector extends towards the sensor in the first path, and light that does not encounter the reflector extends towards the sensor in the second path; and a support structure configured to support a sample in the first path or the second path.

A method and system for performing incoherent color holographic microscopy imaging using light of various wavelengths, including modulating radiation at each wavelength to form two beams and detecting their intensity at a detector. The two beams include phase information that is retrieved from the phase shifted intensity recorded at the detector and holographic information is determined from the detected modulation of the two beams for each color. A processor is configured to receive the holographic information via a signal generated by the detector and the processor further generates a three-dimensional image of a target.

A position tracking system has a laser transmitter, a control tracker and a layout indicator. The laser transmitter has a laser for emitting a laser beam, a controller controlling the laser, and a motor for rotating the emitted laser beam. The control tracker has a housing, at least two photo diodes disposed on the housing, and a laser assembly for generating a beam to be projected unto a surface. The layout indicator has a housing, and at least two photo diodes disposed on the housing. A network server communicates with at least one of the laser transmitter, the control tracker and the layout indicator.

An advance in high-resolution optical metrology has been achieved by the introduction of incoherent holographic imaging. FINCH, an example of incoherent holography, is shown to simplify the process, eliminating many steps in metrology and at the same time increasing throughput, resolution and accuracy of the method. A proposed technique requires only a single image capture with a non-moving camera rather than the capture of multiple stacks of images requiring many camera exposures and movement of the camera or sample in the conventional techniques.

When the optical system is illuminated with an illumination light flux emitted from one extant input image point, an interference image generated by superimposing an extant output light flux output from the optical system and a reference light flux coherent with the extant output light flux is imaged to acquire interference image data, and thus to acquire measured phase distribution, and this acquisition operation is applied to each extant input image point. Thus, each measured phase distribution is expanded by expanding functions μn(u, v) having coordinates (u, v) on a phase defining plane as a variable to be represented as a sum with coefficients Σn{Ajn.Math.μn(u, v)}. When the optical system is illuminated with a virtual illumination light flux, a phase Ψ(u, v) of a virtual output light flux is determined by performing interpolation calculation based on coordinates of a virtual light emitting point.

An apparatus and method to produce a hologram of an object includes an electromagnetic radiation assembly configured to receive a received electromagnetic radiation, such as light, from the object. The electromagnetic radiation assembly is further configured to diffract the received electromagnetic radiation and transmit a diffracted electromagnetic radiation. An image capture assembly is configured to capture an image of the diffracted electromagnetic radiation and produce the hologram of the object from the captured image.