The present invention relates: to a novel transition metal complex having a C—N ligand, which can be used for various devices including an electrochemical sensor, to a device comprising same; and preferably, to an electrochemical sensor.

Disclosed are self-powering biofuel cell and sensor devices, systems and techniques. In some aspects, a self-powered biosensing system includes an electronic circuit; an anode including an enzymatic layer electrically coupled to a power supply voltage terminal of the electronic circuit and configured to interact with an analyte in a fluid, such as glucose or lactate; and a cathode electrically coupled to a ground voltage terminal of the electronic circuit, where the electronic circuit is operable to control and use the electrical energy generated at the anode and cathode for powering the biosensing system and detecting a concentration of the analyte in the fluid.

Methods and systems are described for fabricating thin hydrogel layers on biosensors by a drop-spin method, which includes placing a drop of the hydrogel on the electrode, spinning the wafer at high speed in a vacuum, and heating the wafer to cure. One and multilayer sensors can be fabricated in this way, by adding layers of hydrogel or metal.

The present application discloses a planar enzyme sensor for measuring the concentration of an analyte in a solution comprising a substrate of an electrically insulating material supporting an electrode layer of an electrically conductive material. The substrate and electrode layer have a plurality of layers disposed thereon which include an enzyme layer and a microporous outer layer covering the enzyme layer, wherein the outer layer comprises a continuous phase of a water-resistant polymer (e.g. a polyvinylacetate or an acrylate copolymer), a protein (e.g. an enzyme) embedded in the continuous phase, and possibly polytetrafluoroethylene particles. The enzyme and the polytetrafluoroethylene particles provide a controlled porosity to the outer membrane.

Microfluidic sensing devices utilizing microfluidic sensing electrodes are provided. In one embodiment, a method for fabrication of a microfluidic sensing electrode device for detection of analyte(s) in a fluid is provided, the method comprising: generating a chamber comprising: a tube hole configured to receive a tube, wherein the tube hole extends from a first end of the chamber to a second end of the chamber; and a plurality of electrode holes configured to receive a plurality of electrodes, wherein each of the plurality of electrode holes is in contact with the tube hole; inserting the tube into the tube hole; inserting the plurality of electrodes into the plurality of electrode holes; applying a resin to the chamber; removing the tube from the tube hole, wherein removing the tube from the tube hole exposes a sensing zone that allows the plurality of electrodes to be in contact with the fluid.

An electrochemical microsensor comprising an array of working microelectrodes, the working microelectrodes include: one or more bare microelectrodes; one or more thick film-coated microelectrodes, optionally with conductive additive incorporated into the coating, selected from the group consisting of polysaccharide-coated microelectrodes and platinum black-coated microelectrodes; one or more thin film-coated microelectrodes selected from the group consisting of reduced graphene oxide-coated microelectrode and transition metal chalcogenide-coated microelectrodes; wherein the electrochemical microsensor further comprises a counter electrode and optionally one or more reference microelectrode(s).

Analyte measurement devices and methods of measuring an analyte in a sample. At least one of the methods include: applying an electrical analysis signal to the sample during a measurement time interval (MT), wherein the electrical analysis signal, when transferred into a frequency space, comprises a superposition of two or more non-zero frequency components at least at a sampling time; measuring at least one electrical response signal from the sample; analyzing the electrical response signal; and determining the amount of the analyte in the sample based on the analyzing.

A flow cell with a first section and a second section, and a gasket sealing between the first and second sections. A chamber is defined in the flow cell, having a perimeter with a narrower end and a rounded wider end. An inlet passage, outlet passage, and a sensor are arranged in fluid communication with the chamber. The inlet passage directs fluid into the chamber proximal its narrow end at an angle of between about 45° and 75° relative to the plane of gasket and the outlet passage directs fluid flow out of the wider end of the chamber at an angle between about 45° and 75° relative to the plane of gasket, the inlet passage and outlet passage being angled in opposite directions. The flow cell is useful for monitoring levels of chemicals in an industrial process stream, such as lactose levels in a dairy process stream.

The present disclosure relates to an electrode for measuring an analyte. The electrode includes a first base layer, a first electrode layer upon the first base layer, and a second base layer. The first electrode layer is arranged between the first base layer and the second base layer. The first base layer includes a conductive metal, a conductive metal alloy, or carbon. The first electrode layer includes ruthenium metal, a ruthenium based metal alloy, nickel metal, or a nickel based metal alloy. The first base layer is made of different elements than the first electrode layer. The first base layer is more conductive than the first electrode layer.

Embodiments of the present disclosure relate to electrochemical analyte sensor electrodes that have one or more sensing structures, each structure has a respective perimeter at least partially around it to define the structure so that each of the structures have a liquid limiting barrier around their perimeters. The liquid limiting perimeter may completely or partially encompass the perimeter of each sensing structure of the electrode. Also provided are methods for fabricating the electrodes, analyte sensors employing the subject electrodes, and methods of using the analyte sensors in analyte monitoring.