A microfluidic chip device for the purification of radiochemical compounds includes a chip having an injection channel and intersecting branch channels with a plurality of valves are located along the injection channel and branch channels and configured to retain a plug of solution containing the radiochemical compound. The chip further includes a serpentine channel segment (for separation) coupled to the output of the injection channel. A high voltage power source advances the plug of solution through the purification region and into the downstream fraction collection channel. The chip includes a downstream fraction collection channel coupled to the serpentine channel segment and having an optical and radiation detection regions. One or more branch fraction channels intersect with the fraction collection channel and include valves located therein so that the radiochemical compound that is detected using a radiation detector is directed into the desired branch fraction channel for subsequent use.

A data processor is provided that classifies analysis object data items without performing complex operation in advance. A data processor 1 classifies a plurality of analysis object data items measured by electrophoresis. The data processor 1 includes: a comparison section 8 to perform comparison of the analysis object data items with each other by a predetermined comparison criterion; and a classification section 9 to perform classification of the analysis object data items by a predetermined classification criterion based on a result of the comparison by the comparison section 8 to divide the data items into groups. The comparison section 8 performs the comparison, using the analysis object data items as a reference data item one after another, of the reference data item with each of all the analysis object data items not subjected to the comparison with the reference data item.

An electrophoresis analyzing apparatus includes an acquisition part, an estimation part, and a correction part. The acquisition part acquires actual waveform data on electrophoresis including at least two peak waveforms partially including a superimposed portion. The estimation part estimates, based on an already-appeared peak waveform, a residual portion of an already-appeared peak waveform in the superimposed portion, the already-appeared peak waveform having appeared, in the actual waveform data, before an analysis-target peak waveform to be subjected to waveform analysis. The correction part subtracts the residual portion from the superimposed portion and corrects the analysis-target peak waveform to obtain a true analysis-target waveform.

An electrophoresis analyzing apparatus includes an acquisition part, an estimation part, and a correction part. The acquisition part acquires actual waveform data on electrophoresis including at least two peak waveforms partially including a superimposed portion. The estimation part estimates, based on an already-appeared peak waveform, a residual portion of an already-appeared peak waveform in the superimposed portion, the already-appeared peak waveform having appeared, in the actual waveform data, before an analysis-target peak waveform to be subjected to waveform analysis. The correction part subtracts the residual portion from the superimposed portion and corrects the analysis-target peak waveform to obtain a true analysis-target waveform.

Quality of RNA is evaluated on the basis of a feature value corresponding to a position of a degradation product peak P3 that appears in accordance with degradation of RNA in a region on a low-molecular-weight side relative to an 18S peak P1 in an electrophoresis waveform. The degradation product peak P3 appears in a region on the low-molecular-weight side relative to the 18S peak P1 in accordance with degradation of RNA after RNA has degraded to the extent that the 18S peak P1 disappears, and shifts to the low-molecular-weight side as the RNA degrades. Therefore, even for a degraded RNA, the quality can be evaluated with high accuracy on the basis of the feature value corresponding to the position of this degradation product peak P3.

The invention provides a capillary isoelectric focusing (cIEF) system based on a sandwich injection method for automated chemical mobilization. This system was coupled with an electrokinetically pumped nanoelectrospray interface to a mass spectrometer. The nanoelectrospray emitter employed an acidic sheath electrolyte. To realize automated focusing and mobilization, a plug of ammonium hydroxide was first injected into the capillary, followed by a section of mixed sample and ampholyte. As focusing progressed, the NH.sub.3H.sub.2O section was titrated to lower pH buffer by the acidic sheath buffer. Chemical mobilization started automatically once the ammonium hydroxide was consumed by the acidic sheath flow electrolyte.

In one exemplary embodiment, a method for detecting variants in electropherogram data is provided. The method includes receiving electropherogram data from an instrument and analyzing the electropherogram data to identify mixed bases in the electropherogram data. The method further includes validating the identified mixed bases. Then the method includes determining variants in the electropherogram data based on the validated mixed bases.

An electrophoretic separation data analyzing apparatus includes an analysis target peak identifying part configured to require a user to input analysis target peak information on analysis target peaks to be added to an analysis target in separation data obtained by electrophoretic separation, and to identify at least one of the analysis target peaks based on the analysis target peak information, a peak-of-interest identifying part configured to require the user to input peak-of-interest information on a peak of interest among the analysis target peaks, and identify the peak of interest based on the peak-of-interest information, and an abundance ratio calculating part configured to determine an abundance ratio of the peak of interest among all of the analysis target peaks based on a total value of peak areas of the analysis target peaks and a peak area value of the peak of interest.

The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.

This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.