A method for separating a target substance includes: forming a mixture containing: a target substance-magnetic particle complex that includes: a sample containing a target substance, and magnetic particles to which a first receptor is fixed, wherein the first receptor is adapted to specifically recognize a site of the target substance; and separating the target substance-magnetic particle complex from the mixture by magnetism and electrophoresis.

A Surface-Enhanced Raman Spectroscopy (SERS) device to perform accurate label-free long-read DNA sequencing. A Raman sensor has a hot spot defined by plasmonic nanostructures and excited by at least one laser. An immobilized DNA polymerase can be used to pull a DNA template strand to be sequenced through the hot spot.

A system ensuring the scattering of cells one by one, at a certain speed and direction, on the agarose layer on the lame in SCGE assays is provided. The system includes an electro-magnetic focusing system, a droplet nozzle moving with electro-magnetic guidance, an electric winding creating electromagnetic field for an electro-magnetic charging, an electric motor providing the movement of the lame, an electro-magnetic focusing system, and a control system controlling the electric motor.

The present disclosure provides systems and methods for sample preparation, processing and analysis. Also provided in the present disclosure is a fully-integrated electrophoresis cartridge which has a small footprint and configured to removably engage with the system.

The present disclosure provides systems and methods for sample preparation, processing and analysis. Also provided in the present disclosure is a fully-integrated electrophoresis cartridge which has a small footprint and configured to removably engage with the system.

A magnetohydrodynamic microfluidic system and a method of pumping a fluid using a magnetohydrodynamic system are disclosed. The method includes applying at least one of an electric current and an electric voltage to a first modified electrode and a second electrode to generate an ionic current between the first modified electrode and the second electrode and to cause a current carrying species to move to or from the modified electrode, applying a magnetic field perpendicular to an ionic current vector, the magnetic field and the ionic current combining to induce flow of the fluid in a direction perpendicular to the magnetic field and the ionic current vector, and maintaining fluid flow by recharging the modified electrode.

A magnetic field generation device (100) includes a first magnet (1), a second magnet (2), and a position adjustment mechanism (5). The second magnet (2), together with the first magnet (1), generates a magnetic field. The position adjustment mechanism (5) adjusts a position of the first magnet (1). The magnetic field generation device (100) controls the value of the product of a magnetic flux density and a magnetic flux density gradient in the magnetic field through the adjustment of the position of the first magnet (1) by the position adjustment mechanism (5).

A detection method includes applying a magnetic field to a sample including a composite particle, an unbound particle, and a first solvent, thereby retaining the composite particle and the unbound particle, each of the composite particle and the unbound particle including a magnetic dielectric particle modified by a substance capable of specifically binding to a target substance, the composite particle being bound to the target substance, the unbound particle being not bound to the target substance; replacing, when a predetermined condition is satisfied, at least part of the first solvent with a second solvent with lower electrical conductivity than the first solvent in a state in which the composite particle and the unbound particle are retained; stopping the application of the magnetic field and applying an electric field, thereby separating the composite particle and the unbound particle by dielectrophoresis; and detecting the separated composite particle, thereby detecting the target substance.

Provided herein are methods and systems pertaining to sequencing units of analytes using nanopores. In general, arresting constructs are used to modify an analyte such that the modified analyte pauses in the opening of a nanopore. During such a pause, an ion current level is obtained that corresponds to a unit of the analyte. After altering the modified analyte such that the modified analyte advances through the opening, another arresting construct again pauses the analyte, allowing for a second ion current level to be obtained that represents a second unit of the analyte. This process may be repeated until each unit of the analyte is sequenced. Systems for performing such methods are also disclosed.

In alternative embodiments, the technology described herein is directed in part to combined magnetic tweezer-nanopore devices, in part to combined magnetic tweezer-nanopore sequencing of polymers, and in part to preparation of polymers for combined magnetic tweezer-nanopore sequencing.