The invention relates to a method for producing aminobenzoic acid or a aminobenzoic acid derivative via the fermentation of a suitable raw material under the influence of suitable microorganisms and obtaining a fermentation broth containing aminobenzoate and/or aminobenzoic acid. In particular, the invention relates to the step of obtaining the aminobenzoic acid from the fermentation broth, wherein the crystallisation of aminobenzoic acid is carried out via a simple one-stage acid treatment in the presence of seed crystals. The aminobenzoic acid crystallised in this simple manner can be easily separated from the mother liquor, further cleaned if necessary, and then supplied to the different applications.

The present disclosure relates to a L-glutamic acid-producing recombinant microorganism comprising a SbtA protein or a polynucleotide encoding the SbtA protein, and to a method for producing L-glutamic acid by using the same.

The present invention relates to a Corynebacterium glutamicum mutant strain having increased L-citrulline productivity and a method of producing L-citrulline using the same. The Corynebacterium glutamicum mutant strain is capable of producing L-citrulline in high yield and high concentration because the activity of the transport protein that is expressed by the NCgl2816 gene therein has been weakened or inactivated.

The present disclosure relates to a Corynebacterium glutamicum mutant strain having enhanced L-lysine productivity and a method of producing L-lysine using the same. The Corynebacterium glutamicum mutant strain may produce L-lysine in an improved yield by inhibiting the conversion of oxaloacetate to citrate due to decreased or inhibited expression of the gene encoding the citrate synthase.

A bacterium for producing L-amino acid has improved expression of a polynucleotide encoding a protein represented by SEQ ID NO:3 and improved expression of a polynucleotide encoding a protein represented by SEQ ID NO:31, and/or has mutations in bases at positions −45 bp and −47 bp of a promotor region represented by SEQ ID NO:57. A polynucleotide, encodes proteins and can be included in a recombinant vector, which can be included in a recombinant strain. These are useful in a method for producing L-amino acid. The polynucleotide encodes a protein which is represented by SEQ ID NO:3 and has arginine at position 334 substituted by a terminator or encodes a protein which is represented by SEQ ID NO:31 and has tyrosine at position 592 substituted by phenylalanine, or is formed by mutations in bases at positions −45 bp and −47 bp of a promotor region represented by SEQ ID NO:57.

Provided are a method for introducing point mutations to the coding sequence of NCg12176 gene or improving the expression thereof in Corynebacterium glutamicum, and a method for performing point mutations on the promoter region sequence of dapB gene in Corynebacterium glutamicum. The fermentation yield of L-lysine produced by a strain with the mutations can be increased by means of the methods.

A method of producing 3-oxoadipic acid from an aliphatic compound easily utilizable by a microorganism, such as a saccharide, by utilization of a metabolic pathway of the microorganism is disclosed. The method of producing 3-oxoadipic acid includes the step of culturing at least one type of microorganism having a capacity to produce 3-oxoadipic acid, selected from the group consisting of, for example, microorganisms belonging to the genus Serratia, microorganisms belonging to the genus Corynebacterium, microorganisms belonging to the genus Hafnia, microorganisms belonging to the genus Bacillus, microorganisms belonging to the genus Escherichia, microorganisms belonging to the genus Pseudomonas, microorganisms belonging to the genus Acinetobacter, microorganisms belonging to the genus Alcaligenes, microorganisms belonging to the genus Shimwellia, microorganisms belonging to the genus Planomicrobium, microorganisms belonging to the genus Nocardioides, microorganisms belonging to the genus Yarrowia, microorganisms belonging to the genus Cupriavidus, microorganisms belonging to the genus Rhodosporidium, microorganisms belonging to the genus Streptomyces, and microorganisms belonging to the genus Microbacterium.

The present disclosure relates to a novel protein variant having an activity of exporting 5′-inosine monophosphate, a microorganism comprising the protein variant, and a method for preparing 5′-inosine monophosphate using the microorganism.

Provided are a mutant of a Corynebacterium glutamicum glutamate dehydrogenase gene promoter and applications thereof. The mutant has improved promoter activity compared to a wild-type promoter. Hence, it can be used to enhance the expression of a target gene, for example, operably ligating the mutant with a glutamate dehydrogenase gene, and the expression intensity of the glutamate dehydrogenase can be enhanced, thereby improving the amino acid production efficiency of a recombinant strain.

The present disclosure provides a method for increasing production of an indigoid compound, including the following steps: (a) mutating a wild-type flavin-containing monooxygenase to a mutant flavin-containing monooxygenase expressed in Escherichia coli; and (b) culturing the Escherichia coli in a bacterial culture medium comprising tryptophan to allow the mutant FMO to interact with tryptophan for a predetermined time to convert the tryptophan into the indigoid compound. Compared to the wild-type flavin-containing monooxygenase, the mutant flavin-containing monooxygenase increases production of the indigoid compound. The present disclosure also provides a recombinant polypeptide for increasing production of the indigoid compound.