Disclosed are a mutually independent dual online liquid chromatography device and a control method thereof. The mutually independent dual online liquid chromatography device according to the present disclosure includes a first pump configured to inject a first solvent or a mixed solution containing the first solvent and a second solvent; a second pump configured to inject the first solvent or the mixed solution containing the first solvent and the second solvent; a sample inlet valve to which the first pump and a sample injector configured to inject a sample are connected; a first column valve to which a first sample separation column is connected; a second column valve to which a second sample separation column is connected; and a column selection valve to which the second pump is connected, and interposed between the sample inlet valve, and the first column valve and the second column valve to inject the sample fed from the sample inlet valve onto any one of the first sample separation column and the second sample separation column to separate and analyze the sample in one sample separation column while allowing for wash and equilibration, sample injection and isocratic elution of the other sample separation column.

A device for separating one or more molecules of interest in a liquid specimen including a monolithic body defining a fractionation column. The column includes an inlet opening at a proximal end of the fractionation column; an outlet opening at a distal, opposite end of the fractionation column; a solid phase chamber positioned between the inlet opening and the outlet opening; a specimen introduction area adjacent a proximal end of the solid phase chamber; an analyte exit area adjacent a distal end of the solid phase chamber; an inlet chamber adjacent the inlet opening that tapers into the specimen introduction area; and an outlet chamber that extends from the analyte exit area to the outlet opening. A metered amount of solid phase packed within the solid phase chamber between a first porous frit and a second porous frit of the solid phase chamber.

A method and an apparatus suitable for a continuous chromatography process which only needs three separation columns, and a two-step process containing two chromatographic steps, in which the first chromatographic step (capture) is performed alternating and sequentially on two separation columns, the second chromatographic step (polishing) is performed, also sequentially, on the third column.

Disclosed is a device for chromatographic separations comprising: a manifold comprising a manifold body defining an elongate central duct, the central duct comprising a centrally-located closable duct valve providing selective fluid communication

Go between a first portion of the central duct and an opposed second portion of the central duct, a first plurality of connectors, each connector of the first plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; a second plurality of connectors, each connector of the second plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; wherein said manifold body further defines: a first plurality of branch ducts, each branch duct of which extending from the first portion of the central duct to an individual one of the first plurality of connectors, each of the branch ducts of the first plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the first portion of the central duct, a second plurality of branch ducts, each branch duct of which extending from the second portion of the central duct to an individual one of the second plurality of connectors, each of the branch ducts of the second plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the second portion of the central duct; first and second ports in fluid communication with the centrally-located closable duct valve wherein said first port communicates with said first portion of the central duct and said second port communicates with said second portion of said central duct, wherein one of said first and second ports is further positioned to communicate with said central duct at a location between the centrally-located closable duct valve and the first and second plurality of branch ducts, respectively.

The present disclosure is directed to methods and systems for detecting a substance in a sample gas. The methods and systems include separating the substance of interest in the sample gas, and introducing the separated sample gas into a detector. The systems and methods further include performing an analysis of the substance of interest.

An analysis system is used to together with a sample supplier that supplies a sample, a detector that detects a sample and a display, and includes a plurality of channels arranged in parallel with one another and a display controller that causes a display to display a control screen in regard to control of the plurality of channels. Each channel includes a mobile phase supplier that supplies a mobile phase and an analysis column that separates a sample that has been supplied by the sample supplier into components and guides the components to the detector.

The disclosed system and method improve analysis of chemical samples for measurement of trace volatile chemicals, such as by Gas Chromatography (GC) and Gas Chromatography/Mass Spectrometry (GCMS). The system can include two traps in series, the first of which removes most of the unwanted water vapor, while the second trap preconcentrates the sample using a series of capillary columns of increasing adsorption strength. The sample can be backflushed from the second trap directly to a chemical analyzer without splitting which can maximize sensitivity. The system improves elimination of water vapor and fixed gases from the sample prior to analysis, resulting in detection limits as low as 0.001 PPBb. The second trap allows faster release of the sample upon injection to the chemical analyzer without additional focusing, and can be cleaned up faster when exposed to high concentration samples relative to packed traps.

Disclosed is a device for chromatographic separations comprising: a manifold comprising a manifold body defining an elongate central duct, the central duct comprising a centrally-located closable duct valve providing selective fluid communication between a first portion of the central duct and an opposed second portion of the central duct, a first plurality of connectors, each connector of the first plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; a second plurality of connectors, each connector of the second plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; wherein said manifold body further defines: a first plurality of branch ducts, each branch duct of which extending from the first portion of the central duct to an individual one of the first plurality of connectors, each of the branch ducts of the first plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the first portion of the central duct, a second plurality of branch ducts, each branch duct of which extending from the second portion of the central duct to an individual one of the second plurality of connectors, each of the branch ducts of the second plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the second portion of the central duct; first and second ports in fluid communication with the centrally-located closable duct valve wherein said first port communicates with said first portion of the central duct and said second port communicates with said second portion of said central duct, wherein one of said first and second ports is further positioned to communicate with said central duct at a location between the centrally-located closable duct valve and the first and second plurality of branch ducts, respectively.

Disclosed herein is a packing method for high efficiency chromatography columns starting from dry swellable particles, as well as columns packed by the method and the use of the columns in separation of biomolecules. In the packing method, an amount of dry swellable particles sufficient to give a swollen volume in a liquid of about 105-120% of the column chamber volume is transferred to the column, the column is closed and the liquid is provided to the column.

Disclosed is a device for chromatographic separations comprising: a manifold comprising a manifold body defining an elongate central duct, the central duct comprising a centrally-located closable duct valve providing selective fluid communication between a first portion of the central duct and an opposed second portion of the central duct, a first plurality of connectors, each connector of the first plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; a second plurality of connectors, each connector of the second plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; wherein said manifold body further defines: a first plurality of branch ducts, each branch duct of which extending from the first portion of the central duct to an individual one of the first plurality of connectors, each of the branch ducts of the first plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the first portion of the central duct, a second plurality of branch ducts, each branch duct of which extending from the second portion of the central duct to an individual one of the second plurality of connectors, each of the branch ducts of the second plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the second portion of the central duct; first and second ports in fluid communication with the centrally-located closable duct valve wherein said first port communicates with said first portion of the central duct and said second port communicates with said second portion of said central duct, wherein one of said first and second ports is further positioned to communicate with said central duct at a location between the centrally-located closable duct valve and the first and second plurality of branch ducts, respectively.