A CuV.sub.2O.sub.6-based photoelectric sensor is prepared through the following steps: acquiring a CuV.sub.2O.sub.6 thin film through a direct-current reactive magnetron co-sputtering method; and loading an 8-hydroxyquinoline solution on the CuV.sub.2O.sub.6 thin film through a spin-coating method to acquire an 8-hydroxyquinoline-modified CuV.sub.2O.sub.6 photoelectric sensor. The 8-hydroxyquinoline-modified CuV.sub.2O.sub.6 photoelectric sensor has a good anti-interference capability in the detection of arginine; it is easy to realize the low-cost mass production of CuV.sub.2O.sub.6 photoelectrodes through a developed direct-current reactive magnetron sputtering coating method; and a sensor device is low in cost, simple, portable, and easy to use, and has an application value in food safety and health and hygiene detection.

A method includes mounting an integrated electro-microfluidic probe card to a device area on a bio-sensor device wafer, wherein the electro-microfluidic probe card has a first major surface and a second major surface opposite the first major surface. The method further includes electrically connecting at least one electronic probe tip extending from the first major surface to a corresponding conductive area of the device area. The method further includes stamping a test fluid onto the device area. The method further includes measuring via the at least one electronic probe tip a first electrical property of one or more bio-FETs of the device area based on the test fluid.

Provided herein are devices (e.g., electrochemical sensors useful for detecting volatile organic compounds associated with certain diseases or conditions and/or diagnosing certain diseases or conditions). The devices comprise one or more layers of metal on a layer of silicon, and a layer of molecularly imprinted polymer in electrical communication with the one or more layers of metal, wherein the one or more layers of metal are each independently selected from a layer of chromium, platinum, gold, nickel, cobalt, tungsten, rhodium, iridium, silver, tin, titanium or tantalum, or an alloy thereof. Methods of using the devices (e.g., to detect one or more analytes in a sample, to detect and/or diagnose a disease or condition in a subject), and methods of making the devices are also provided.

An electrochemical sensor arrangement (10) for a breath alcohol measuring device (100), to a corresponding breath alcohol measuring device (100) as well as to a process for determining a vitality of electrodes of an electrochemical sensor. The electrochemical sensor arrangement comprises an electrochemical sensor with at least two electrodes (12, 14). The electrochemical sensor arrangement further comprises a heat source (16). The heat source is arranged such that it, upon activation, selectively heats one of the electrodes (12) of the electrochemical sensor.

Disclosed in the embodiments of the present application are a microelectrode of a gene sequencing chip, a manufacturing method therefor, and a gene sequencing chip. The microelectrode comprises a substrate, a current collector layer formed on the substrate, and an electrode layer formed on the current collector layer; the current collector layer comprises a transition metal thin film or a nitride thin film thereof or a composite thin film of a transition metal and nitride thereof, and the electrode layer comprises a nitrogen oxide thin film of the transition metal, which is formed on the transition metal thin film or the nitride thin film thereof or the composite thin film of the transition metal and nitride thereof The embodiments of the present application improve the per unit area voltage driving capabilities of a microelectrode in a gene sequencing chip, can meet requirements for an ultra-high number of cycles, and improve the throughput and stability of a gene sequencing chip.

A CRISPR electrochemical biosensing system (E-CRISPR) for detection of analytes includes a disposable, micro-fabricated three-electrode sensor that includes a working electrode, a counter electrode, a reference electrode, and a nonspecific ssDNA reporter with an electrochemical tag for signal transduction tethered to a surface of the working electrode; and a Cas12a-crRNA duplex that is designed to specifically recognize and cleave target nucleic acid strand based on the protospacer adjacent motif (PAM) sequence of the target and crRNA sequence, wherein the PAM recognition depends on specific 5′ TTTN nucleic acid sequence located at an opposite strand of a recognition strand, and wherein only upon the recognition of the PAM sequence by the Cas protein, the Cas protein, acting as a DNA helicase, unwinds the target DNA.

Methods for detecting one or more analytes in a sample utilizing Electrochemical Impedance Spectroscopy (EIS) measurement. In one method, analyte detection includes comparing an imaginary impedance measurement to a calibration curve of concentrations for each target analyte. The calibration curve of concentrations for each target analyte is established at an optimal frequency. In another method, a signal decoupling algorithm is utilized for detection of more than one analyte on an electrode.

A portable biosensor for detecting and quantifying a target molecule in a biological sample and method of use include a biosensor fabricated with a recognition layer with an imprinted polymer, an electrode electrically coupled to the recognition layer, and a logic circuit that may include a processor and non-transitory memory with computer executable instructions embedded thereon, wherein the imprinted polymer is shaped to have a profile that substantially matches a profile of the target molecule, such that the target molecule can form-fit and bind to the imprinted polymer, thus changing an electrical property of the polymer layer that may be detected to identify the presence of the target molecule.

In a general aspect, an apparatus can include a first carbon nanotube array that is patterned to define a first electrode having a first plurality of electrode segments. The apparatus can also include a second carbon nanotube array that is patterned to define a second electrode having a second plurality of electrode segments. The second plurality of electrode segments can be interdigitated with the first plurality of electrode segments. The apparatus can further include a biorecognition agent disposed on a surface of the first electrode and disposed on a surface of the second electrode. The first plurality of electrode segments can each have a height-to-width aspect ratio of at least 1 to 1.

Provided is a preparation method for an immunoelectrode. The immunoelectrode comprises a substrate, a gold layer, a conductive polymer layer and an antibody layer. The substrate, the gold layer, the conductive polymer layer and the antibody layer are sequentially attached from bottom to top. The preparation method for the immunoelectrode specifically comprises the following steps: (1) preparing the conductive polymer layer: preparing a polypyrrole layer on a gold-plated substrate to obtain a polypyrrole/gold-plated substrate; (2) preparing the immunoelectrode: preparing the antibody layer on the polypyrrole layer to obtain an antibody/polypyrrole/gold-plated substrate; and (3) forming an immunoelectrode system: fixing a bare gold-plated substrate to the outer side of the antibody/polypyrrole/gold-plated substrate to obtain the immunoelectrode system. A polypyrrole material is used for fixing an antibody of a biological recognition element and immobilizing the antibody on the immunoelectrode.