The present invention provides pathogen-inactivated red blood cell compositions.

The present invention provides pathogen-inactivated red blood cell compositions.

Provided herein are materials and methods relating to cell-free DNA. In particular, the technology relates to methods and materials for the preparation and handling of blood samples for future use in applications involving cell-free DNA.

An aqueous stabilizing composition for preserving a bodily fluid at ambient temperature is provided. The aqueous stabilizing composition comprises: a sugar selected from a monosaccharide, a disaccharide, or a combination thereof; a buffering agent; a C.sub.1-C.sub.6 alkanol; boric acid, a salt of boric acid, or a combination thereof; and a chelating agent; wherein the composition has a pH of from 4.5 to 5.2. A method for preserving a bodily fluid using the aqueous stabilizing composition is also provided, the method comprising: a) obtaining a sample of the bodily fluid; b) contacting the bodily fluid with the aqueous stabilizing composition to form a mixture; c) mixing the mixture of (b) to form a homogeneous mixture; and d) storing the homogeneous mixture at ambient temperature.

Provided are systems and methods for treating a biological fluid, e.g., to inactivate pathogens.

This disclosure relates to methods of preserving mesenchymal stromal/stem cells (MSCs) for use in clinical applications. In certain embodiments, this disclosure relates to methods of preserving MSCs comprising mixing MSCs with interferon-gamma prior to cryopreserving, freezing, or cooling the MSCs to a temperature below zero degrees Celsius.

Relates to a method for pathogen reduction of a blood product comprising: i) removing oxygen from a blood product; ii) reducing blood pathogens from said blood product t: adding amustaline (S-303) to a final concentration of 0.2 millimolar (mM); and glutathione (GSH) to a concentration of 20 mM; iii) reducing S-303 to a concentration of less than 1 nmol/L comprising incubating said oxygen reduced blood product under oxygen reduced conditions for up to 6 hours; and further relates to a pathogen reduced, oxygen reduced blood product having an oxygen saturation (sO.sub.2) of less than 25%, a pCO.sub.2 of 90 mmHg or less at 37° C., and having a amustaline (S-303) concentration of less than 1 nmol/L.

Provided are systems and methods for treating a biological fluid, e.g., to inactivate pathogens.

The present invention is directed to methods for collecting and processing autografts, processed autografts, kits for collecting and transporting autografts, and tools for preparing autografts. It is also directed to autologous bone grafts, and methods of preparing them.

A composite material film for expanding circulating tumor cells ex vivo and a preparation method thereof, a kit and a method for expanding circulating tumor cells ex vivo, a method for detecting an effect of a drug, and a cryopreservation solution are provided. The preparation method includes: mixing one or more kinds of particles and a solvent to form a mixed liquid, in which the particles are selected from the group consisting of metal particles, metal oxide particles, silicon oxide particles and combinations thereof; placing the mixed liquid on a substrate to form a particle layer; adding a medium material to the particle layer, in which the medium material is selected from the group consisting of styrene and its derivatives, polyester monomers, silicon oxide compounds and combinations thereof; and polymerizing the medium material to form a medium layer to fix the particle layer on the substrate.