The present application relates to a method for detecting and monitoring the level of thyroid hormones in an individual and a device for carrying out the same.

The embodiments disclose an apparatus including a test cartridge configured for inserting into a reader, a sample insertion component coupled to the test cartridge a test sample, a heater device coupled to the test cartridge configured to heat to a predetermined temperature the test sample, a sensor array coupled to the test cartridge consisting of at least one electrochemical sensor for sensing analytes in a sample, a reader configured to gather test related data from the sensor array coupled to the test cartridge, an analyzer coupled to the reader configured for determining test results of each sensor in the sensor array and comparing all the test results for confirmation of a valid test and coherent results, a MCU (Microcontroller Unit) to perform main control and processing functions, and orchestrates all the functionality for the Reader, and a BLE Radio RF link between the MCU and an external processing system.

A lateral flow assay device comprises a test strip to receive a quantity of fluid comprising a quantity of exosomes and detect the presence of a target analyte on the surface of the exosomes. The test strip comprises a conjugate pad that contains a set of one or more types of tetraspanin binding reagents conjugated with a label. Each type of tetraspanin binding reagent is configured to bind with a corresponding type of exosome tetraspanin and form an immunocomplex comprising an exosome. The conjugate pad is fluidly connected to a membrane. The membrane comprises a test line comprising an immobilized binding reagent to the target analyte. The immobilized binding reagent to the target analyte is configured to bind to a protein of the target analyte on the surface of an exosome in an immunocomplex comprising the exosome.

The present invention relates to an interdigitated microelectrode biosensor using the reaction between receptors and target biomaterials, the interdigitated microelectrode biosensor comprising: an insulating layer formed so as to cover all of the sensor formation area of a substrate; a first interdigitated microelectrode formed such that a plurality of first protruding electrodes are arranged in a comb shape on the insulating layer of the substrate; a second interdigitated microelectrode, facing the first interdigitated microelectrode and formed such that a plurality of second protruding electrodes are arranged in a comb shape on the insulating layer of the substrate such that the plurality of second protruding electrodes are arranged to respectively interdigitate with the plurality of first protruding electrodes formed at the first interdigitated microelectrode; and a plurality of receptors arranged in the space between the first and second interdigitated microelectrodes, which are arranged to interdigitate with each other, so as to specifically react with the target biomaterial, thereby increasing an impedance detection width and detection limit, and improving detection accuracy according to the characteristics of each monomer and each polymer.

Methods and devices for quantifying creatinine in a test sample are provided. The test sample is contacted with a sensing composition to obtain a product comprising a hydantoin-transition metal complex and ammonia. The sensing composition comprises creatinine deaminase and a transition metal salt. The creatinine deaminase enzymatically reacts with creatinine to provide the N-methyl hydantoin and ammonia. The N-methyl hydantoin forms the hydantoin-transition metal complex with the transition salt. A potential difference is applied to the product to measure a current signal provided by the hydantoin-transition metal complex. Concentration of N-methyl hydantoin is obtained based on the measured current signal using a calibration equation. The concentration of N-methyl hydantoin is correlated with concentration of creatinine to quantify the creatinine in the test sample.

An electrode composed of a substrate including a graphene layer coated on a first metal; and a complex including a second metal deposited on the substrate and a hydroxide of the first metal, where the complex is in the form of core-shell in which the second metal is a core and the hydroxide of the first metal is a shell, and the second metal has a higher standard reduction potential than the first metal. The graphene-coated metal foam of the present invention is the first case that proves not only theoretically but also by experiment that the remarkable catalytic ability reducing other metals (Au, Pt, Ag, and Cu, etc.) with a higher reduction potential than the metal by graphene coated on the metal surface it electroless deposition without additional reductant or electrical reduction conditions is due to the electrical double layer or interfacial dipole induced between the graphene and the metal.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P).sup.+) or derivative thereof; an NAD(P).sup.+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

A biosensor, including a modified gold electrode and a macrophage RAW264.7 immobilized on the modified gold electrode. The disclosure also provides a method of preparing the biosensor and a method of using the same for evaluation of antioxidant capacity of substances.

Provided are an amadoriase that is less likely to be influenced by oxygen concentration and a method and a reagent kit for measurement of HbA1c using such amadoriase. Provided are an amadoriase that is obtained via substitution of one or more amino acid residues at a position or positions corresponding to the position(s) selected from the group consisting of positions 280, 267, 269, 54, and 241 of the amadoriase derived from the genus Coniochaeta, a method for measurement of HbA1c, a reagent kit for measurement, and a sensor using such amadoriase. The modified amadoriase according to the invention has a lowered oxidase activity and an enhanced dehydrogenase activity, and this enables the use of an electron mediator, and this reduces the influence of oxygen concentration. Thus, HbA1c can be measured with high sensitivity.

A peptide-imprinted conductive polymer and use thereof is provided, especially a peptide-imprinted conductive polymer including conductive polymer monomer(s), two-dimensional (2D) material(s), and a small peptide fragment of α-synuclein as template. The peptide-imprinted conductive polymer has high sensibility, detects α-synuclein at low concentrations, thus allowing early diagnosis and treatment of Parkinson's disease.